| 湖南典型茶树地理种群遗传多样性研究 |
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| 引用本文： | 沈程文,黄意欢,黄建安,罗军武,刘春林,刘德华.湖南典型茶树地理种群遗传多样性研究[J].农业生物技术学报,2007,15(5). |
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| 作者姓名： | 沈程文 黄意欢 黄建安 罗军武 刘春林 刘德华 |
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| 作者单位： | 湖南农业大学园艺园林学院 教育部茶学重点实验室 作物基因工程湖南省重点实验室 |
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| 摘 要： | 本文利用RAPD分子标记技术，对4个湖南典型茶树地理种群的240个单株的遗传多样性、种群内和种群间的遗传变异进行研究，结果表明：21个10碱基随机引物共检测到226条谱带，其中多态性谱带为201条，占88.9%。遗传多样性分析结果显示：Shannon's多样性指数为0.43，74.7%的变异分布于群体内，而种群间变异占了25.3%；Nei's指数群体总基因多样度(HT)为0.37，群体内平均基因多样度(HS)0.28，群体间的基因多样度(HST)0.09，群体Nei's基因分化系数（GST）为0.23，说明76.7%的变异存在于种群间，群体内的变异占了总变异的23.3%，与Shannon's多样性指数相比基本一致，均表明种群内有较丰富的遗传变异；种群间的基因流（Nm）为0.74，显示种群间的基因交流有限。
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| 收稿时间： | 2007-3-19 |
| 修稿时间： | 2007-5-31 |
RAPD analysis on genetic diversity of typical tea populations in Hunan Province |
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| Abstract: | The genetic diversity and genetic variation within and among four tea populations in Hunan were studied by RAPD markers. The result showed that 226 loci were detected by using 21 random primers (10bp) of which 162 loci were polymorphic (88.9%). Species genetic diversity indicated by Shannon's index was 0.43, 74.7% of which was due to within population genetic diversity and 25.3% among population variation. Species gene diversity indicated by Nei's index was 0.37, gene diversity within populations (HS) was 0.28, gene diversity among populations (HST) was 0.09 and the coefficient of gene differentiation (GST) among the populations was 0.23, 76.7% of which was within populations and 23.3% was among populations. The gene diversity estimated by Nei's index was consistent with that estimated by Shannon's index. These results suggest that there is rich genetic within population variation, which offers excellent prospects for seeding selection. The estimate of gene flow from GST (Nm) was 0.74, which indicated quite small genetic recombination between populations, it probably is related to environmental adaptations of four tea populations in Hunan and population isolation in their high mountain habitat. |
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