| 紫花苜蓿与蓝花苜蓿抗旱性比较研究 |
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| 引用本文： | 陈静,宫文龙,张尚雄,韦宝,王赞,黄琳凯.紫花苜蓿与蓝花苜蓿抗旱性比较研究[J].中国草地学报,2020(2):32-40. |
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| 作者姓名： | 陈静 宫文龙 张尚雄 韦宝 王赞 黄琳凯 |
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| 作者单位： | 四川农业大学动物科技学院;中国农业科学院北京畜牧兽医研究所;中国农业大学动物科学技术学院 |
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| 基金项目： | 国家现代农业产业技术体系(CARS34);国家自然科学基金(31761143013)。 |
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| 摘 要： | 试验采用两次反复干旱法在三叶期对77份紫花苜蓿及蓝花苜蓿材料进行干旱胁迫,以正常浇水为对照,于第二次胁迫后调查植株存活率、株高、地上生物量、地下生物量、根长及根冠比等6项形态指标。对各指标抗旱系数进行方差、相关性、主成分及聚类分析,以揭示紫花苜蓿及蓝花苜蓿材料对干旱胁迫的响应及抗旱性差异。结果表明,在紫花苜蓿与蓝花苜蓿材料中,地下生物量均为抗旱系数差异最大的指标,最大值与最小值之间的差异分别为4.07倍(紫花苜蓿)和2.11倍(蓝花苜蓿)。株高的抗旱系数在不同亚种及种质间差异均达到极显著水平,且与存活率、地上生物量及根冠比间均存在显著相关性,表明株高在鉴定苜蓿抗旱性的6个有效指标中效果最明显。主成分分析可将6个形态指标分别归类为地上部性状和地下部性状,且可根据前两个主成分因子将紫花苜蓿和蓝花苜蓿清晰地划分为两个抗旱性不同的独立类群。聚类结果将77份材料划分为抗旱特征不同的5个亚组,可根据不同研究目的进行选择利用。综上,依据6个形态指标对紫花苜蓿及蓝花苜蓿进行抗旱性评价的结果为四倍体紫花苜蓿的抗旱性要强于二倍体蓝花苜蓿,试验结果对多倍体逆境胁迫研究和紫花苜蓿抗旱育种研究具有重要意义。
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| 关 键 词： | 紫花苜蓿 蓝花苜蓿 多倍体 干旱胁迫 抗旱性 |
Comparative Study on Drought Resistance of Two Alfalfa Subspecies |
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| CHEN Jing,GONG Wen-long,ZHANG Shang-xiong,WEI Bao,WANG Zan,HUANG Lin-kai.Comparative Study on Drought Resistance of Two Alfalfa Subspecies[J].Chinese Journal of Grassland,2020(2):32-40. |
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| Authors: | CHEN Jing GONG Wen-long ZHANG Shang-xiong WEI Bao WANG Zan HUANG Lin-kai |
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| Institution: | (Animal Science and Technology College,Sichuan Agricultural University,Chengdu 611130,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | A repeated drought rehydration method was applied to 77 trefoil-staged alfalfa germplasms of both subspecies(regularly watered samples as the control group). Six morphological parameters(survival rate, plant height, aboveground biomass, underground biomass, root length, and root-stem ratio) were measured after the second drought stress test. To reveal the response to drought and the differences in drought resistance, a series of analyses were performed including variance analysis, correlation analysis, principal component analysis, and cluster analysis. The results showed that underground biomass was the most significant variable in both diploid and tetraploid alfalfa germplasms, in which the differences between the maximal and the minimal were 4.07(Medicago sativa ssp. sativa) and 2.11(M. sativa ssp. caerulea), respectively. There was a significant difference in plant height among all germplasms of respective subspecies, which also had significant correlations with survival rate, aboveground biomass, and roof-top ratio, indicating that plant height was the most relevant parameter to draught stress among all six parameters. The principal component analysis classified six morphological parameters into two categories, including aboveground and underground traits, which divided all germplasms of the two species into groups with different draught tolerances. The cluster analysis divided all 77 germplasms into five sub-groups with different draught tolerances, which could be selected for specific research purposes. In conclusion, draught tolerances of autotetraploid ssp. sativa was better than those of diploid ancestor ssp. caerulea based on the evaluation of 6 morphological parameters. This finding is important to study the breeding of drought-tolerance polyploidy alfalfa. |
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| Keywords: | Medicago sativa ssp sativa M sativa ssp caerulea Polyploid Drought stress Drought resistance |
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