| 稀碱预处理对杂交狼尾草木质纤维素降解的影响 |
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| 引用本文： | 高凤芹,陶雅,吴洪新,王慧,景媛媛,张晓庆.稀碱预处理对杂交狼尾草木质纤维素降解的影响[J].中国草地学报,2020(1):58-67. |
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| 作者姓名： | 高凤芹 陶雅 吴洪新 王慧 景媛媛 张晓庆 |
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| 作者单位： | 中国农业科学院草原研究所 |
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| 基金项目： | 国家自然科学基金项目(31802134);内蒙古自治区科技计划项目(201802082);中国农业科学院科技创新工程项目(CAAS-ASTIP-IGR 2015-02)。 |
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| 摘 要： | 木质纤维素结构复杂,且不同植物间差异较大,预处理技术不尽相同。为分析稀碱预处理对草类植物木质纤维素的降解情况,以杂交狼尾草为原料,利用氢氧化钠溶液进行预处理,分析碱液浓度、固液比、处理时间和温度4个单因素对杂交狼尾草木质纤维素降解的影响;在此基础上,采用L8(2^4)正交试验,确定主要影响因素,并筛选最佳条件组合。结果表明,影响稀碱预处理杂交狼尾草降解因素的主次顺序为:浓度>固液比>温度>时间,其中碱液浓度的增加促进半纤维素和木质素的降解,产生更多的水解糖;固液比的增加在一定范围内(1:8~1:10)可显著影响木质素和半纤维素的降解(P<0.05);预处理时间的延长并不能显著改善纤维素和半纤维素的降解率,100℃是影响木质素脱除的重要临界点,继续增加温度无显著性影响。杂交狼尾草稀碱预处理的最佳工艺条件是:氢氧化钠浓度3.0%、固液比1:8、时间15min及温度120℃。最佳条件预处理后杂交狼尾草木质纤维素结构发生塌陷,非纤维物质被显著脱除,纤维束清晰可见,纤维素可保留74.48%,半纤维素和木质素脱除率达77.17%和90.62%。
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| 关 键 词： | 杂交狼尾草 木质纤维素 稀碱预处理 最佳条件 |
Effect of Dilute Alkali Pretreatment on Lignocellulose Degradation of Hybrid Pennisetum |
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| GAO Feng-qin,TAO Ya,Wu Hong-xin,WANG Hui,JING Yuan-yuan,ZHANG Xiao-qing.Effect of Dilute Alkali Pretreatment on Lignocellulose Degradation of Hybrid Pennisetum[J].Chinese Journal of Grassland,2020(1):58-67. |
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| Authors: | GAO Feng-qin TAO Ya Wu Hong-xin WANG Hui JING Yuan-yuan ZHANG Xiao-qing |
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| Institution: | (Institute of Grassland Research of Chinese Academy of Agricultural Sciences,Hohhot 010010,China) |
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| Abstract: | The structure of lignocellulose is complex,and there are great differences among different plants,and the pretreatment technology is different.In order to analyze the degradation of lignocellulose in hybrid Pennisetum by dilute alkali pretreatment,sodium hydroxide solution was used to pretreat the lignocellulose in hybrid Pennisetum.The effects of four single factors,namely alkali concentration,solid-liquid ratio,treatment time and temperature,on the degradation of lignocellulose in hybrid Pennisetum were analyzed.The main influencing factors were determined by L8(2^4)orthogonal test,and the optimum technological combination was screened.The results showed that the sequence of factors affecting degradation of hybrid Pennisetum by dilute alkali pretreatment was as follows:first concentration,secondly solid-liquid ratio,the third is temperature,and time is the last.The increase of alkali concentration positively promoted the degradation of hemicellulose and lignin and produced more hydrolyzed sugar,and the increase of solid-liquid ratio in a certain range(1:8-1:10)could significantly affect the degradation of lignin and hemicellulose(P<0.05).The degradation rate of cellulose and hemicellulose could not be significantly improved by prolonging the time.Temperature of 100℃ was the critical point for lignin removal,but there was no significant effect if the temperature continued to increase.The optimum technological conditions for dilute alkali treatment of hybrid Pennisetum were as follows:NaOH concentration 3.0%,solid-liquid ratio 1∶8,time 15min,temperature 120℃.The structure of lignocellulose collapsed after pretreatment under the optimum technological conditions,and the non-cellulose substances were removed significantly,the cellulose bundles were clearly visible.After the pretreatment,the cellulose could be retained by 74.48%,the removal rates of hemicellulose and lignin were 77.17% and 90.62%. |
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| Keywords: | Hybrid Pennisetum Lignocellulose Dilute alkali pretreatment Optimum conditions |
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