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Characterization of shiga toxin-producing Escherichia coli strains isolated from calves in Poland |
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| Authors: | Osek J Gallien P Protz D |
| Institution: | a National Veterinary Research Institute (PIWet.), Partyzantow 57, 24-100 Pulawy, Poland b Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jahnstrasse 8, 06846 Dessau, Germany c Diedersdorfer Weg 1, 12277 Berlin, Germany |
| Abstract: | Fecal samples from 67 3–5-months-old calves with diarrhea were screened for the presence of shiga toxin-producing Escherichia coli (STEC). Several accessory virulence factors genes were also tested. Among 192 E.coli isolates tested, 15 (7.6%) were found to harbour the shiga toxin 1 or 2 (stx1 or stx2) genes. The stx2-carrying samples were further subtyped by PCR for the stx2c, stx2d, and stx2e toxin variants. It was shown that stx2-positive bacteria mainly possessed the stx2c shiga toxin type gene. The enterohemolysin (hlyA) and intimin (eae) genes were found in seven (46.7%) STEC strains whereas the cytotoxic necrotizin factor 1 and 2 or the P fimbrial genes were detected in two isolates only. This study confirmed that calves are a reservoir of STEC strains (with all pathogenicity genes) that may be virulent for humans. |
| Keywords: | E coli Calves Diarrhea Shiga toxin Pathogenicity factors PCRAuthor Keywords: E coli Veaux Diarrhée Toxine Shiga Facteurs de pathogenité PCR |
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