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| 猪繁殖与呼吸障碍综合征病毒ORF5基因的克隆与表达 | |
| 引用本文： | 田小辉,王爱萍,乔松林,张改平,肖治军,冯丽丽,刘运超,李乔木,李卓阳.猪繁殖与呼吸障碍综合征病毒ORF5基因的克隆与表达[J].河南农业科学,2009(2). |
| 作者姓名： | 田小辉 王爱萍 乔松林 张改平 肖治军 冯丽丽 刘运超 李乔木 李卓阳 |
| 作者单位： | 1. 郑州大学,生物工程系,河南,郑州,450001;河南省农业科学院,河南省动物免疫学重点实验室,河南,郑州,450002 2. 郑州大学,生物工程系,河南,郑州,450001 3. 河南省农业科学院,河南省动物免疫学重点实验室,河南,郑州,450002 |
| 摘 要： | 用RT-PCR方法从河南分离的猪繁殖与呼吸障碍综合征病毒(PRRSV)变异株(HN07-1)中扩增ORF5全长基因,将其克隆至pMD19-T载体。测序结果表明,该序列与标准美洲株VR-2332同源性为89%,与我国早期分离的BJ-4株同源性为88%,与HB-1、JXAI变异株同源性为96%和99%。将该基因亚克隆到原核表达载体pGEX-6p-1后,转化大肠杆菌BL21,IPTG诱导表达。SDS-PAGE和Western-Blot结果表明,成功表达了41.5 kD的融合蛋白。 |
| 关 键 词： | 猪繁殖与呼吸障碍综合征病毒 变异株 ORF5 原核表达 |
Cloning and Expression of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) |
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| TIAN Xiao-hui,WANG Ai-ping,QIAO Song-lin,ZHANG Gai-ping,XIAO Zhi-jun,FENG Li-li,LIU Yun-chao,LI Qiao-mu,LI Zhuo-yang.Cloning and Expression of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV)[J].Journal of Henan Agricultural Sciences,2009(2). | |
| Authors: | TIAN Xiao-hui WANG Ai-ping QIAO Song-lin ZHANG Gai-ping XIAO Zhi-jun FENG Li-li LIU Yun-chao LI Qiao-mu LI Zhuo-yang |
| Institution: | 1.Department of Bioengineering;Zhengzhou University;Zhengzhou 450001;China;2.Henan Provincial Key Laboratory of Animal Immunology;Henan Academy of Agricultural Sciences;Zhengzhou 450002;China |
| Abstract: | The full-length of ORF5 gene was amplified from the genomic RNA of Henan variant PRRSV strain HN07-01 by RT-PCR.The amplified fragment was cloned into pMD19-T vector and then sequenced.Compared with the ORF5 gene sequences of BJ-4,classical American genotype VR-2332,HB-1 and JXA-1,the homology of HN07-01 ORF5 gene was 88%,89%,96% and 99%,respectively.The ORF5 gene was subcloned into the prokaryotic expression vector pGEX-6p-1,transformed into E.coli BL21 and induced with IPTG.The results of SDS-PAGE and Wes... |
| Keywords: | ORF5 |
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