| 大豆黄酮对伊莎鸡卵泡发育及其P450arom基因表达的影响 |
| |
| 引用本文： | 刘红云,周彩琴,曾卫东,张才乔.大豆黄酮对伊莎鸡卵泡发育及其P450arom基因表达的影响[J].中国畜牧杂志,2010,46(17). |
| |
| 作者姓名： | 刘红云 周彩琴 曾卫东 张才乔 |
| |
| 基金项目： | 国家自然科学基金，浙江省科技厅项目 |
| |
| 摘 要： | 实验探讨了大豆黄酮(DAI)对伊莎鸡卵泡发育及其芳香化酶(P450arom)mRNA表达的影响。实验选取16只产蛋后期伊莎鸡,等分为对照组和DAI处理组。对照组饲喂基础日粮,实验组在基础日粮中添加10 mg/kgDAI。实验持续7周后,分离排卵前卵泡(F1、F2、F3……)的颗粒层及小黄卵泡和大白卵泡,通过RT-PCR法检测P450arom mRNA表达的相对丰度。结果表明:DAI明显提高了伊莎鸡小黄卵泡和大白卵泡的数量,P450arommRNA在伊莎鸡不同发育阶段卵泡中的表达存在差异,部分卵泡P450arom mRNA表达的相对丰度显著增加。因此,在产蛋后期伊莎鸡基础日粮中添加DAI可增加不同发育阶段卵泡的数目,上调部分卵泡中与发育相关的基因表达以促进卵泡发育。
|
| 关 键 词： | 大豆黄酮 卵泡发育 芳香化酶 伊莎鸡 |
Effects of Daidzein on Follicle Development and mRNA Expression of P450arom in Ovarian Follicles of Isa Hens |
| |
| LIU Hong-yun,ZHOU Cai-qin,ZENG Wei-dong,ZHANG Cai-qiao.Effects of Daidzein on Follicle Development and mRNA Expression of P450arom in Ovarian Follicles of Isa Hens[J].Chinese Journal of Animal Science,2010,46(17). |
| |
| Authors: | LIU Hong-yun ZHOU Cai-qin ZENG Wei-dong ZHANG Cai-qiao |
| |
| Abstract: | Effects of DAI on follicle development and follicle development-related gene(P450 aromatase(P450arom)) mRNA expressions in ovarian follicles of Isa hens were evaluated.The hens were 13 months old in the post-peak period of egg laying and were randomly allocated into control and daidzein-treated groups,with daidzein supplemented to the basal diet at 10 mg/kg for 7 consecutive weeks.The mRNA expression of related genes was measured by semi-quantitative RT-PCR in the granulosa layers of the preovulatory follicle(POF: F1,F2 …) and follicular layers of the small yellow follicle(SYF),large white follicle(LWF) and atretic follicle(ATF).Results showed that daidzein supplementation significantly increased the number of SYF and LWF(P < 0.05).P450arom mRNA was the highest in the SYF,but was very low in the granulosa layers after follicles finished selection.Treatment with daidzein resulted in increased mRNA expression of P450arom in LWF(P < 0.05).These results indicated that dietary supplementation of daidzein stimulated follicle develop-ment and up-regulated mRNA expression of P450arom to improve the development of preovulatory follicles in Isa hens after the peak-laying period. |
| |
| Keywords: | daidzein follicle development P450 aromatase(P450arom) Isa hens |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
