|
|||||
|
|
| 云南羊肚菌rDNA的ITS序列与亲缘关系分析 | |
| 引用本文： | 沈洪,陈明杰,赵永昌,潘迎捷.云南羊肚菌rDNA的ITS序列与亲缘关系分析[J].食用菌学报,2007,14(2):15-22. |
| 作者姓名： | 沈洪 陈明杰 赵永昌 潘迎捷 |
| 作者单位： | 1. 上海农业科学院食用菌研究所,上海市农业遗传育种重点开放实验室,上海,201106;南京农业大学,南京,210095 2. 上海农业科学院食用菌研究所,上海市农业遗传育种重点开放实验室,上海,201106 3. 云南省农业科学院生物技术资源研究所,昆明,650205 4. 上海水产大学,上海,200090 |
| 摘 要： | 采用ITS(Internal Transcribed Spacers)序列比对技术分析了根据形态挑选的14个羊肚菌子实体(其中11个来自云南,3个来自浙江)。结果表明,云南的11个羊肚菌子实体(M1~M11)可分为4个种:高羊肚菌(Morchella elata),尖顶羊肚菌(Morchella conica),粗柄羊肚菌(Morchella crassipes)和大孔(宽肋)羊肚菌(Morchella costata)。浙江的3个羊肚菌子实体(M12~M14)经鉴定为未知羊肚菌种。14个羊肚菌子实体样品和GenBank中黑脉(小顶)羊肚菌Morchella angusticeps(AJ698476)]、美味羊肚菌Morchella esculenta(AJ698475)]以及羊肚菌科常见属钟菌属Verpa conica(AJ544206)的ITS序列聚类,以钟菌(AJ544206)作为外类群,可分为三大聚类群:高羊肚菌、大孔(宽肋)羊肚菌和黑脉(小顶)羊肚菌为第1类群(Group1);美味羊肚菌和尖顶羊肚菌为第2类群(Group2);粗柄羊肚菌和浙江的3个羊肚菌为第3类群(Group3)。 |
| 关 键 词： | 羊肚菌 Internal Transcribed Spacers序列 相似性 |
| 文章编号： | 1005-9873（2007）02-0015-04 |
| 收稿时间： | 2006-11-10 |
| 修稿时间： | 2006-11-102007-02-14 |
Classification of Morchella species from Yunnan and Zhejiang Provinces Based on rDNA ITS Sequences |
|
| SHEN Hong,CHEN Mingjie,ZHAO Yongchang,PAN Yingjie.Classification of Morchella species from Yunnan and Zhejiang Provinces Based on rDNA ITS Sequences[J].Acta Edulis Fungi,2007,14(2):15-22. | |
| Authors: | SHEN Hong CHEN Mingjie ZHAO Yongchang PAN Yingjie |
| Institution: | 1 Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2 Microbiology Department, Nanjing Agriculture University, Nanjing,Jiangsu 201195, China ; 3.yunnan Institute of Biological Technologies, Yunnan Agricultural Academy, Kunming, Yunnan 650205, China; 4.Shanghai Fisheries University, Shanghai 200090, China |
| Abstract: | Fourteen Morchella samples (eleven from Yunnan and three from Zhejiang Provinces) were selected on the basis of differences in fruit body morphology. Ribosomal DNA internal transcribed spacers (ITS) were amplified in each case using the universal primer pair, ITS-1 and ITS-4, and the amplification products were purified and sequenced. Comparisons with sequence data in GenBank revealed that the 11 Morchella isolates collected from Yunnan belonged to four species: Morchella elata, Morchella conica, Morchella crassipes and Morchella costata. The three isolates collected from Zhejiang Province (M12, M13 and M14) were designated as unknown Morchella species. When Verpa conica (AJ544206) (from the genus Verpa belonging to the same family as Morchella) was taken as the outgroup, the 14 isolates formed three groups, M. elata, M. costata (Group 1); Morchella esculenta, M. conica (Group 2); and M. crassipes, M12, M13 and M14 (Group 3). |
| Keywords: | Morchella ITS sequences Similarity coefficient |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |