Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids |
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| Authors: | Veli-Matti Rokka Yong-Sheng Xu Jyri Kankila Anja Kuusela Seppo Pulli Eija Pehu |
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| Institution: | (1) Agricultural Research Centre, Institute of Crop and Soil Science, Plant Breeding Section, FIN-31600 Jokioinen, Finland;(2) Department of Plant Production, University of Helsinki, P.O. Box 27, FIN-00014 Viikki, Finland;(3) Agricultural Research Centre, Institute of Plant Protection, FIN-31600 Jokioinen, Finland |
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| Abstract: | Summary Symmetric somatic hybrids were produced by electrofusion of protoplasts of two dihaploid tuber-bearing potato (Solanum tuberosum L.) lines and Solanum brevidens Phil., a diploid non-tuber-bearing wild potato species. A total of 985 plants was obtained. Verification of nuclear hybridity of putative hybrids was based on additive RAPD patterns, general morphological characteristics and chromosome counts. 53 (90%) calli regenerated into plants which were identified as somatic hybrids. Most of the hybrids were aneuploids at the tetraploid (4×) or hexaploid (6×) level. The 20 hybrids tested expressed a high level of resistance to potato virus Y (PVY
N
) characteristic of the S. brevidens parent. Resistance to late blight (Phytophthora infestans (Mont.) de Bary) varied between hybrids, but was on average better than that of the fusion parents. Resistance of hybrids to bacterial stem rot (Erwinia carotovora subsp. atroseptica (van Hall) Dye) was not superior to that of commercial potato cultivars. |
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| Keywords: | Erwinia carotovora subsp atroseptica Phytophthora infestans PVY RAPD Solanum brevidens Solanum tuberosum somatic hybrids |
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