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| 凡纳滨对虾BTF3基因的克隆及表达分析 | |
| 引用本文： | 殷勤,彭金霞,崔亮,谢达祥,王志伟,李奎,陈晓汉.凡纳滨对虾BTF3基因的克隆及表达分析[J].西南农业学报,2011,24(6). |
| 作者姓名： | 殷勤 彭金霞 崔亮 谢达祥 王志伟 李奎 陈晓汉 |
| 作者单位： | 1. 广西壮族自治区水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁530021;中国农业科学院北京畜牧兽医研究所,北京100094 2. 广西壮族自治区水产研究所,广西水产遗传育种与健康养殖重点实验室,广西南宁530021 3. 中国农业科学院北京畜牧兽医研究所,北京,100094 |
| 基金项目： | 广西壮族自治区直属公益性科研院所基本科研业务费(2060302GXIF-2010-04); 现代农业产业技术体系建设专项资金(编号:CARS-47); 广西科学研究与技术开发计划(桂科攻10100007-2); 国家科技支撑计划项目(2008BADB9B03) |
| 摘 要： | |
| 关 键 词： | 凡纳滨对虾 BTF3 基因克隆 表达分析 |
Molecular Cloning and Expression Analysis of BTF3 in Litopenaeus vannamei |
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| YIN Qin , PENG Jin-Xia , CUI Liang , XIE Da-xiang , WANG Zhi-Wei , LI Kui , CHEN Xiao-Han.Molecular Cloning and Expression Analysis of BTF3 in Litopenaeus vannamei[J].Southwest China Journal of Agricultural Sciences,2011,24(6). | |
| Authors: | YIN Qin PENG Jin-Xia CUI Liang XIE Da-xiang WANG Zhi-Wei LI Kui CHEN Xiao-Han |
| Institution: | YIN Qin1,2,PENG Jin-Xia1,CUI Liang1,XIE Da-xiang1,WANG Zhi-Wei2,LI Kui2,CHEN Xiao-Han1*(1.Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Guangxi Institute of Fisheries,Guangxi Nanning 530021,China,2.Institute of Animal Sciences(IAS),Chinese Academy of Agricultural Sciences(CAAS),Beijing 100094,China) |
| Abstract: | A pair of primers were designed according to the sequence from electronic cloning,and a 655 bp cDNA of BTF3 in Litopenaeus vannamei was first obtained by RT-PCR.The cDNA contained a 597 bp open reading frame(ORF) encoding a 198 amino acids peptide.The molecular weight of BTF3 was 45.79 kDa,and its calculated isoelectric point was 4.93.Sequence alignment revealed that the amino acid sequence of BTF3 from different species were highly conserved and contained a NAC domain.Real-time PCR results showed that BTF3... |
| Keywords: | Litopenaeus vannamei BTF3 Gene cloning Expression analysis |
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