| 氮胁迫条件下稻瘟病菌分泌蛋白与水稻悬浮细胞互作中“活性氧”变化分析 |
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| 引用本文： | 周晓罡,侯思名,苏源,陶南,丁玉梅,姚春馨,孙茂林,张绍松.氮胁迫条件下稻瘟病菌分泌蛋白与水稻悬浮细胞互作中“活性氧”变化分析[J].西南农业学报,2011,24(6). |
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| 作者姓名： | 周晓罡 侯思名 苏源 陶南 丁玉梅 姚春馨 孙茂林 张绍松 |
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| 作者单位： | 1. 云南省农业科学院生物技术与种质资源研究所,云南昆明650223;云南省农业生物技术重点实验室,云南昆明650223
2. 昆明大学生命科学与技术学院,云南昆明,650031 |
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| 基金项目： | 国家自然科学基金项目(31060021); 云南省自然科学基金项目(2006C0062M,2008ZC100M) |
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| 摘 要： | 以CH-63和TH-16 2个致病性差异显著的稻瘟病菌株为材料,在氮胁迫培养条件下进行液体培养,经培养2d后,采用铵盐沉淀及透析纯化提取稻瘟病菌培养滤液中的致病性分泌蛋白.以24个IRRI水稻抗稻瘟病单基因系和普感品种丽江新团黑谷(LTH)的成熟种胚为材料,诱导愈伤组织并构建水稻悬浮细胞系.以构建成功的感病品种LTH和抗病品种IRBL-9的水稻悬浮细胞系为材料,接种稻瘟病菌致病性分泌蛋白并对活性氧浓度变化进行检测.结果表明,感病与抗病品种在接种前12 h均出现活性氧迸发,且均出现2个峰值.比较稻瘟病菌株TH-16和CH-63分泌蛋白接种IRBL-9与LTH后H2O2浓度变化特征,发现分泌蛋白致病性强弱与H2O2浓度变化呈正相关,CH-63产生的强致病性分泌蛋白引起活性氧浓度的快速增加.TH-16产生的弱致病性分泌蛋白接种IRBL-9与LTH后活性氧浓度则增加不明显.上述研究对于深入了解稻瘟病菌与水稻之间的相互识别、信号传导和应答过程,阐明水稻对稻瘟病菌的抗性机制、水稻与稻瘟病菌互作的分子机理具有重要意义.
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| 关 键 词： | 氮胁迫 分泌蛋白 致病性 悬浮细胞系 活性氧 |
Changes in Concentration of Active Oxidative Analysis of Rice Blast Secreted Protein and Rice Suspension Cell Interaction under Nitrogen Starvation |
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| ZHOU Xiao-gang
,
HOU Si-ming
,
SU Yuan
,
TAO Nan
,
DING Yu-mei
,
YAO Chun-Xin
,
SUN Mao-lin
,
ZHANG Shao-song.Changes in Concentration of Active Oxidative Analysis of Rice Blast Secreted Protein and Rice Suspension Cell Interaction under Nitrogen Starvation[J].Southwest China Journal of Agricultural Sciences,2011,24(6). |
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| Authors: | ZHOU Xiao-gang HOU Si-ming SU Yuan TAO Nan DING Yu-mei YAO Chun-Xin SUN Mao-lin ZHANG Shao-song |
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| Institution: | ZHOU Xiao-gang1,2,HOU Si-ming3,SU Yuan1,TAO Nan1,DING Yu-mei1,YAO Chun-Xin1,SUN Mao-lin1,2*,ZHANG Shao-song1,2(1.Biotechnology and Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650223,China,2.Key Laboratory of Agricultural Biotechnology of Yunnan Province,3.College of Life Science and Technology,Kunming University,Yunnan Kunming 650031,China) |
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| Abstract: | The rice blast fungal strains CH-63 and TH-16 with 2 different pathogenic factors were cultivated in a liquid culture medium under nitrogen starvation.After 2 d of cultivation,the protein secreted by the fungal strains was extracted by ammonium sulfate sedimentation and purified by dialysis.Mature embryos of the above mentioned 24 monogenic rice lines and universally susceptible japonica rice variety Lijiangxintuanheigu(LTH) were used as materials to induce callus in inducing medium I and II;the callus-indu... |
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| Keywords: | Nitrogen starvation Secreted protein Pathogenicity Cell suspension culture Active oxidative |
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