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Random amplified polymorphic DNA variation among German and Dutch asparagus cultivars
Authors:T Jaag    A Langsdorf    R J Snowdon    W Köhler  H D Hartmann
Institution:Biometrie und Populationsgenetik, Justus-Liebig-Universität Giessen, Ludwigstrasse 27, D-35392 Giessen, Germany;Forschungsanstalt Geisenheim, Rebenweg 8, D-65366 Geisenheim, Germany
Abstract:DNA polymorphism among nine cultivars of Asparagus officinalis L. was measured using random amplified polymorphic DNA (RAPD). Of 69 reproducible amplification products from 12 arbitrary decamer primers, 49 RAPD markers were polymorphic and could be used to distinguish six German and three Dutch asparagus cultivars. Even with very small sample sizes, genetic similarity measurements based on the RAPD data allowed accurate grouping of the nine cultivars into distinct clusters, with the exception of two individuals which clustered to closely related varieties. Two German cultivars showed high genetic similarity and were distinct from the remaining German varieties. The German and Dutch cultivars were clearly separated by a relatively large genetic distance.
Keywords:Asparagus officinalis            cultivar identification  RAPD polymorphism
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