| Tae-miR9677小串联模拟靶标(STTM)表达载体构建及小麦的遗传转化研究 |
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| 引用本文： | 李赵杰,简 超,刘香利,赵惠贤.Tae-miR9677小串联模拟靶标(STTM)表达载体构建及小麦的遗传转化研究[J].麦类作物学报,2016,36(4):404-408. |
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| 作者姓名： | 李赵杰 简 超 刘香利 赵惠贤 |
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| 作者单位： | 西北农林科技大学生命科学学院/旱区作物逆境生物学国家重点实验室,陕西杨陵,712100 |
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| 基金项目： | 国家自然科学基金项目（31471482，31101205） |
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| 摘 要： | 小串联模拟靶标(Short tandem target mimic,STTM)技术是一种新开发的miRNA功能研究方法。Tae-miR9677作为一种新发现的在小麦穗部特异性高表达的miRNA,其功能至今未知。为了进一步探索Tae-miR9677的功能,构建了Ubiqutin(UBI)启动子启动的Tae-miR9677 STTM过表达载体,并通过基因枪介导法对小麦品种绵阳19幼胚愈伤组织进行转化。结果表明,3 683个愈伤组织经过PPT(Phosphinothricin)筛选,最终分化获得42株再生植株;利用特异性引物进行PCR检测,鉴定出8株T0代阳性植株。
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| 关 键 词： | Tae-miR9677 小串联模拟靶标(STTM) 小麦 载体构建 转基因 |
Construction of Expression Vector of Tae-miR9677 Short Tandem Target Mimic (STTM) and Transformation in Wheat |
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| LI Zhaojie,JIAN Chao,LIU Xiangli,ZHAO Huixian.Construction of Expression Vector of Tae-miR9677 Short Tandem Target Mimic (STTM) and Transformation in Wheat[J].Journal of Triticeae Crops,2016,36(4):404-408. |
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| Authors: | LI Zhaojie JIAN Chao LIU Xiangli ZHAO Huixian |
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| Abstract: | Short tandem target mimic (STTM) is an important method for miRNA function research. Tae-miR9677 is a newly discovered miRNA with high expression level in wheat spike,and its function has not been revealed. Therefore,it is very important to explore the function of Tae-miR9677 using STTM technology. In this investigation,the plant expression vector of Tae-miR9677 STTM controlled under Ubiqutin promoter was constructed. Then,3 683 immature embryos of Mianyang 19 were transformed using this vector by biolistic particle. After phosphinothricin selection and regeneration,42 regenerated plants were obtained,and of which eight were identified to be positive transgenic plants with Bar and Tae-miR9677 genes by PCR analysis. |
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| Keywords: | Tae-miR9677 Short tandem target mimic (STTM) Wheat Construction of expression vector Transformation |
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