| 小麦L699叶片受白粉菌胁迫后蛋白质的差异表达 |
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| 引用本文： | 梁银萍,常小丽,张 敏,龚国淑,雷 雨,祁小波,罗丽雅,胡玉亭.小麦L699叶片受白粉菌胁迫后蛋白质的差异表达[J].麦类作物学报,2016,36(4):460-464. |
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| 作者姓名： | 梁银萍 常小丽 张 敏 龚国淑 雷 雨 祁小波 罗丽雅 胡玉亭 |
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| 作者单位： | 四川农业大学农学院,四川成都,611130 |
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| 基金项目： | 国家公益性行业（农业）科研专项（201303016） |
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| 摘 要： | 为从蛋白组学角度认识小麦抗白粉病机制,以含有抗白粉病新基因Pm40的小麦品系L699为材料,采用蛋白质双向电泳和质谱技术(MALDI-TOF-MS)检测小麦叶片接种白粉菌24h后的差异蛋白。结果表明,经PDQuest软件分析,接种白粉菌后小麦叶片中有18个蛋白质斑点表达量上调,38个蛋白质斑点表达量下调。对表达量上调的蛋白质斑点进行质谱分析和数据库检索,共鉴定出15种蛋白质,其中13种参与防御反应、碳水化合物代谢和蛋白质周转。这些差异蛋白与抗病途径、植株生理过程密切相关,在小麦抗白粉病过程中起很重要的作用。
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| 关 键 词： | 小麦 白粉菌 Pm40 蛋白质 双向电泳 |
Differential Proteomics Analysis of Wheat Line L699 Induced by Blumeria graminis |
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| LIANG Yinping,CHANG Xiaoli,ZHANG Min,GONG Guoshu,LEI Yu,QI Xiaobo,LUO Liy,HU Yuting.Differential Proteomics Analysis of Wheat Line L699 Induced by Blumeria graminis[J].Journal of Triticeae Crops,2016,36(4):460-464. |
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| Authors: | LIANG Yinping CHANG Xiaoli ZHANG Min GONG Guoshu LEI Yu QI Xiaobo LUO Liy HU Yuting |
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| Abstract: | The aim of this study was to investigate the defense-related proteins of wheat line L699 against Blumeria graminis and to provide some basis for the resistance mechanism of wheat-Blumeria graminis through proteomic analysis. Proteins were extracted from the wheat leaf at 24 h after inoculation and analyzed by two dimensional electrophoresis and MALDI-TOF-MS. Results demonstrated that as compared to non-inoculated plants, expression of 18 proteins spots significantly increased after Blumeria graminis inoculation on the gel stained with coomassie brilliant blue G250, whereas 38 proteins spots decreased. Furthermore, 15 up-regulated proteins were identified by MALDI-TOF-MS and analyzed using the NCBInr database of Triticum and these identified proteins were predicted to be associated with defense response, carbohydrate metabolism, and protein turnover. It is suggested that the proteins are not only involved in disease defense response but also in other physiology-related pathway in the process that wheat resists to the Bgt. |
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| Keywords: | Wheat Blumeria graminis Pm40 Protein Two-dimensional electrophoresis |
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