To select suitable organic substrate block for the seedling culture of hot pepper by studying the effect of different organic substrates on the growth of hot pepper seedlings. Hot pepper seeds were sowed in two different organic substrate blocks (J1, J2) and one plug tray with organic substrate (J3). The period of emergency, emergency rate, seedling height, leaf area and chlorophyll content were measured. The period of emergence of hot pepper in the plug tray was one day earlier than that in the two organic substrate blocks, and there were no significant differences in emergence rate between J1 and J3, but the emergence rate in them was significantly higher than that in J2 (P<0.01). It also showed that J1 was more favorable for the growth of hot pepper seedlings because the plant height, leaf area, leaf quantity, chlorophyll content of seedlings were obviously higher than that in J2 and J3 (P<0.01). J1 was a kind of organic substrate block strengthening pepper seedlings in culture.