In order to understand the characteristic of nitrogen uptake and utilization of grafting cultivation on cucumber yield, the relationship between cultivation methods and fertilization on cucumber yield, experiment was conducted using split spot design that cucumber ‘Cuilv’ was the scion and pumpkin ‘Qingyan stock No.1’ was the rootstocks. The results showed that cucumber yield reached after grafting 60380.18 kg/hm2, compared with 62.02% increase from the root cultivation. Grafting cultivation and fertilization treatments were significant improve cucumber nitrogen uptake were 32.44% and 28.13%, nitrogen use efficiency and nitrogen use efficiency was no significant difference.