In order to know the relationship between suture softening of peach fruit and ethylene, experiment was carried out with 1-MCP (1-methylcyclopropene) treating the peach fruit at growth period, using gas chromatography to determine ethylene level in fruit, observing the rate of fruit suture softening and influence of 1-MCP on leaf and fruit quality. The results showed that, 1-MCP could effectively reduce the ethylene levels in suture site and kernel of peach fruit, reduce the embryo abortion with 80% compared with control; reduced suture softening fruit ratio, which was only 2% while the control was high up to 23%; adverse effects of 1-MCP on fruit quality and leaves was not found. So that 1-MCP can not only inhibit the ethylene occurs in fruit during postharvest storage, but also can inhibit the ethylene occurred in fruit during growth. It indicated that this can provide reference to 1-MCP applications for pre-harvest production.