| 枯草芽孢杆菌WB600芽孢和营养体耐受凡纳滨对虾胃肠道环境及定殖存活的研究 |
| |
| 引用本文： | 吴正存,傅玲琳,李卫芬,励建荣.枯草芽孢杆菌WB600芽孢和营养体耐受凡纳滨对虾胃肠道环境及定殖存活的研究[J].水产科学,2011,30(6):311-316. |
| |
| 作者姓名： | 吴正存 傅玲琳 李卫芬 励建荣 |
| |
| 作者单位： | 1. 浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室,浙江杭州310035
2. 浙江大学动物科学学院,生物饲料安全与污染防控国家工程实验室,浙江杭州310029 |
| |
| 基金项目： | 浙江工商大学青年人才基金资助项目，浙江省自然科学基金资助项目，浙江工商大学研究生科研创新基金资助项目 |
| |
| 摘 要： | 采用体外模拟胃肠道环境与体内芽孢存活计数相结合方法,考察枯草芽孢杆菌WB600耐受凡纳滨对虾胃、肠道环境及其在凡纳滨对虾肠道内的定殖存活情况。试验结果表明,枯草芽孢杆菌WB600芽孢在不同的pH环境中均表现出很强的耐受性和良好的生长增殖趋势,而营养体的耐受性较差;在含不同浓度胰蛋白酶的模拟肠液中芽孢和营养体均能表现出良好的耐受性,其中芽孢的耐受能力更为显著;当温度高达80℃时,仍有93.9%的芽孢存活,而营养体基本失活;在虾的肠道内容物中能检测到大量的活芽孢,说明芽孢在进入虾肠道内是可以在其内存活并生长定殖的。表明枯草杆菌WB600芽孢比营养体对胃肠道环境和高温具有更强的耐受能力,且能顺利在虾肠道内定殖存活,有望在水产养殖领域发挥益生作用的同时成为新型的口服疫苗载体。
|
| 关 键 词： | 枯草芽孢杆菌 芽孢 凡纳滨对虾 胃肠道环境 高温 定殖存活 |
The Resistance and Survival of Bacillus subtilis within Gastrointestinal Environment of Pacific White Leg Shrimp (Litopenaeus vannamei) |
| |
| WU Zheng-cun,FU Ling-lin,LI Wei-fen,LI Jian-rong.The Resistance and Survival of Bacillus subtilis within Gastrointestinal Environment of Pacific White Leg Shrimp (Litopenaeus vannamei)[J].Fisheries Science,2011,30(6):311-316. |
| |
| Authors: | WU Zheng-cun FU Ling-lin LI Wei-fen LI Jian-rong |
| |
| Institution: | 1(1.Food Safety Key Laboratory of Zhejiang Province,School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310035,China;2.National Engineering Laboratory of Biological Feed Safety and Pollution Control,College of Animal Sciences,Zhejiang University,Hangzhou 310029,China) |
| |
| Abstract: | To evaluate the resistance of probiotic Bacillus subtilis in the gastrointestinal tract environment of shrim Pacific white Leg,the counts compared to original inocula were determined after treatment of vegetative cells and spores in the simulated gastric juice and midgut fluid.Moreover,the colonization and survival in the spores in vivo of shrimp was also studied.The results showed that spores well-survived and proliferated in the simulated gastric juice with pH 2.0~3.0,which demonstrated the higher resistance than that in vegetative cells.Both vegetative cells and spores resisted the simulated midgut fluid of the shrimp.The percent of surviving spores was 93.9% after treatment at a temperature of 80 ℃,while the vegetative cells were almost inactivated at this condition.The spores were extremely robust with most colonization and survival in the shrimp intestine.The conclusion was that the B.subtilis WB600 spores with higher resistance against the simulated gastrointestinal condition and high temperature survived and colonized in vivo in shrimp.These key features make this bacterium effective as probiotics and in the safe delivery of antigens across the gastrointestinal barrier when it was applied in aquaculture. |
| |
| Keywords: | Bacillus subtilis WB600 spore shrimp(Litopenaeus vannamei) gastrointestinal environment high temperature colonization and survival |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
