Genetic diversity analysis of two buffalo populations of northern India using microsatellite markers |
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Authors: | R Arora B D Lakhchaura R B Prasad M S Tantia R K Vijh |
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Institution: | Animal Genetics Division, NBAGR, Karnal, Haryana;, Animal Biotechnology Centre, College of Veterinary Science, GBPUA&T, Pantnagar, Uttaranchal;, Genetics and Animal Breeding Division, College of Veterinary Sciences, GBPUA&T, Pantnagar, Uttaranchal;, and Animal Genetic Resources Division, NBAGR, Karnal, Haryana, India |
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Abstract: | The genetic diversity in two buffalo populations of northern India, the Bhadawari and the Tarai was assessed using a set of 22 heterologous (bovine) microsatellite markers. The average number of alleles across all loci in both populations was found to be 4.7, indicating that this set of 22 bovine microsatellite markers could be used to study genetic variation in buffalo species also. The overall polymorphic information content (PIC) value for these markers was 0.54. The average observed and expected heterozygosities for both populations were 0.59 and 0.64, respectively. Common alleles with varying allele frequencies in both populations also represented the genetic variability existing between Bhadawari and Tarai buffaloes. However the θ estimates for population differentiation indicated low levels of differentiation between the two populations. This was further supported by the low genetic distance (0.155) between Bhadawari and Tarai, which was calculated using Nei's standard genetic distance method. The present study on Bhadawari and Tarai populations represents a much‐needed preliminary effort that could be extended to other local buffalo populations of India as well. |
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