The Differentiation-and Proliferation-Inhibitory Effects of Sporamin from Sweet Potato in 3T3-L1 Preadipocytes |
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| Authors: | XIONG Zhi-dong LI Peng-gao MU Tai-hua |
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| Institution: | [1]Laboratory of Food Chemistry and Nutrition Science, Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China [2]Department of Nutrition and Food Hygiene, School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, P.R. China |
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| Abstract: | The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-L1 preadipocytes,providing the theoretical basis for the development of food to treat obesity and diabetes.The isolation and purification of sporamin from sweet potato species 55-2 were performed by ammonium sulphate precipitation in combination with ion-exchange and gel filtration chromatography.With berberine as a positive control,different concentrations of sporamin (0.000,0.125,0.025,0.250,0.500,and 1.000 mg mL-1) were used to treat 3T3-L1 preadipocytes.Intracellular fat accumulation and the degree of adipogenesis were quantified using Oil Red O staining and colorimetry.Preadipocytes differentiation was measured by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT)spectrophotometric assay.Two sporamin proteins,which were separated into sporamin A (31 kD) and sporamin B (22 kD),could be purified by ion-exchange and gel filtration chromatography.After being treated by different concentrations of sporamin,the differentiation of 3T3-L1 preadipocytes was significantly inhibited,compared with the positive control.When the sporamin solution concentration was 0.500 mg mL-1,the accumulation of lipid droplets within the cells was significantly decreased and the optical density (OD) value of the solution from destained Oil Red O reached to 0.35,which was the lowest value (P ＜ 0.05).The proliferation of 3T3-L1 preadipocytes was significantly inhibited by treating at higher sporamin concentrations.In addition,the inhibitory effect was more obvious with the prolonged treatment time (P＜ 0.05).The differentiation and proliferation of 3T3-L1 preadipocytes could be inhibited significantly by the addition of higher concentration sporamin.It was,therefore,suggested that the sporamin was potentially effective for weight loss. |
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| Keywords: | sweet potato sporamin 3T3-L1 preadipocytes |
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