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| 邓恩桉继代增殖培养条件研究初报 | |
| 引用本文： | 易霭琴,童方平,宋庆安,李贵,丁婕,唐春.邓恩桉继代增殖培养条件研究初报[J].中国农学通报,2007,23(12):154-157. |
| 作者姓名： | 易霭琴 童方平 宋庆安 李贵 丁婕 唐春 |
| 作者单位： | 1. 湖南省林业科学院,长沙,410004;湖南省林木无性系育种重点实验室,长沙,410004 2. 中南林业科技大学,长沙,410004 3. 湖南省湘潭县林业局,湘潭,411228 |
| 摘 要： | 为提高邓恩桉组培苗的增殖系数,降低组培苗生产成本,获得健壮的邓恩桉组培苗,通过不同基本培养基、谷氨酸、培养基不同pH值以及不同光照强度对邓恩桉继代培养增殖的影响试验,找到了邓恩桉的组织培养中继代增殖的适宜条件。结果表明,基本培养基、谷氨酸、pH值和光照强度对试管苗的继代增殖都会产生不同程度的影响。基本培养基以改良H为好,添加20mg/L的谷氨酸有利于试管苗的生长,pH值6.2最为适合,最合适的光照强度为2500lx。因此,适合邓恩桉继代增殖的培养条件为:改良H BA0.2～0.4mg/L NAA0.1～0.2mg/L IBA0.2mg/L LH500mg/L 谷氨酸20mg/L,pH6.2,光照强度2500lx。 |
| 关 键 词： | 邓恩桉 组织培养 继代 增殖系数 培养条件 |
| 收稿时间： | 2007-08-24 |
| 修稿时间： | 2007-09-26 |
Studies on the Culture Conditions of Eucalyptus dunnii Subculture and Multiplication |
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| Yi Aiqing,Tong Fangping,Song Qingan,Li Gui,Ding Jie,Tang Chun.Studies on the Culture Conditions of Eucalyptus dunnii Subculture and Multiplication[J].Chinese Agricultural Science Bulletin,2007,23(12):154-157. | |
| Authors: | Yi Aiqing Tong Fangping Song Qingan Li Gui Ding Jie Tang Chun |
| Abstract: | In order to improve the multiplication coefficient of Eucalyptus dunnii, reduce the cost of culture and obtain the vigorous test-tube plantlet, culture conditions were optimized through testing the multiplication rate and height of test-tube plantlet under different conditions such as basic media, glutamic acid (Glu), pH, intensity of illumination. Through above research, this culture conditions influenced the subculture and multiplication of test-tube plantlet. Basic medium had effects on subculture, modified H was better than H, MS and modified MS. The culture media with Glu promoted the growth far more effectively than non- Glu ones. pH 6.2 and 2500 lx were the most suitable pH and intensity of illumination. So, the suitable culture conditions of Eucalyptus dunnii were modified H BA 0.2 mg/L NAA 0.1 mg/L IBA 0.2 mg/L LH 500 mg/L Glu20mg/L, pH 6.2, intensity of illumination 2500 lx. |
| Keywords: | Eucalyptus dununii tissue culture subculture multiplication culture conditions |
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