Assessment of Genetic Diversity among Finger Millet (<Emphasis Type="Italic">Eleusine coracana</Emphasis> (L.) Gaertn.) Accessions using Molecular Markers |
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| Authors: | B Kalyana Babu N Senthil S Michael Gomez K R Biji N S Rajendraprasad S Satheesh Kumar R Chandra Babu |
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| Institution: | (1) Department of Plant Molecular Biology and Biotechnology, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, 641003 Coimbatore, India;(2) Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, 641003 Coimbatore, India |
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| Abstract: | Finger millet (Eleusine coracana), an allotetraploid cereal, is widely cultivated in the arid and semiarid regions of the world. Being rich in protein and
calcium, finger millet serves as an important staple food for rural populations in developing tropical countries where calcium
deficiency and anemia are wide spread. Thirty-two finger millet genotypes were fingerprinted using 50 random amplified polymorphic
DNA (RAPD) markers. Out of the total 529 loci generated using the 50 RAPD primers, 479 loci (91%) were polymorphic and informative
to differentiate the accessions. Cluster analysis grouped the 32 finger millet accessions into two major clusters. Among the
32 finger millet genotypes, GEC 182 and CO 12 were distantly related with a low similarity index of 0.315. These two accessions
also differed considerably in days to flowering and grain weight; GEC 182 is early flowering and has bold grains, while CO
12 is late flowering and has smaller grains. These two accessions with higher diversity at molecular level, phenology and
grain weight will be ideal as parents in hybridization programme, to develop improved finger millet varieties suitable for
peninsular region of India. |
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| Keywords: | Eleusine coracana Finger millet Genetic diversity Jaccard’ s similarity coefficient RAPDs |
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