| 壳寡糖季铵盐衍生物纳米银的合成及其诱导烟株抗烟草花叶病毒活性 |
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| 引用本文： | 孙翠红,徐翠莲,赵铭钦,黄元炯,张露,尤方芳,周伏叶.壳寡糖季铵盐衍生物纳米银的合成及其诱导烟株抗烟草花叶病毒活性[J].农药学学报,2015,17(4):391-400. |
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| 作者姓名： | 孙翠红 徐翠莲 赵铭钦 黄元炯 张露 尤方芳 周伏叶 |
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| 作者单位： | 1.河南农业大学 烟草学院国家烟草栽培生理生化研究基地, 郑州 450002 |
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| 基金项目： | 河南省烟草公司重点科技攻关项目(HYKJ201015,HNNKY2013-062). |
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| 摘 要： | 为开发符合高效、低毒且环境友好的新型烟草花叶病毒(TMV)抑制剂,以具有季铵盐结构的新型壳寡糖季铵盐衍生物为还原剂和稳定剂,合成了壳寡糖季铵盐衍生物纳米银溶液,并研究了不同浓度该纳米银溶液诱导烟株产生抗TMV的活性。结果表明:所合成的壳寡糖季铵盐衍生物纳米银溶液在透射电镜(TEM)下显示分布较均匀,粒径主要集中在7~12 nm。在珊西烟上以25μg/m L的壳寡糖季铵盐衍生物纳米银溶液对枯斑的抑制效果最好,抑制率为74.0%,比50μg/m L壳寡糖溶液和2%宁南霉素水剂分别高41.5%和24.4%;对于普通烟K326,壳寡糖季铵盐衍生物纳米银溶液可缓解被病毒侵染的烟草叶绿素含量的下降幅度,提高叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(PPO)的活性,降低丙二醛(MDA)的含量,提高可溶性蛋白含量。表明壳寡糖季铵盐纳米银溶液可提高烟株对TMV的抗病性。
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| 关 键 词： | 壳寡糖季铵盐衍生物 纳米银 合成 烟草花叶病毒 防御酶 |
| 收稿时间： | 2014/11/2 0:00:00 |
| 修稿时间： | 2015/4/19 0:00:00 |
Synthesis of silver nanoparticles using quaternized oligochitosan derivative and its induced resistance against TMV in tobacco plant |
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| Sun Cuihong,Xu Cuilian,Zhao Mingqin,Huang Yuanjiong,Zhang Lu,You Fangfang and Zhou Fuye.Synthesis of silver nanoparticles using quaternized oligochitosan derivative and its induced resistance against TMV in tobacco plant[J].Chinese Journal of Pesticide Science,2015,17(4):391-400. |
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| Authors: | Sun Cuihong Xu Cuilian Zhao Mingqin Huang Yuanjiong Zhang Lu You Fangfang and Zhou Fuye |
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| Institution: | 1.National Tobacco Cultivation & Physiology & Biochemistry Research Center, College of Tobacco, Henan Agricultural University, Zhengzhou 450002, China2.College of Science, Henan Agricultural University, Zhengzhou 450002, China3.Tobacco Leaf Company of Henan Province, Zhengzhou 450002, China |
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| Abstract: | In order to develop a new type of inhibitors of tobacco mosaic virus (TMV), which is efficient, low toxic and environmentally friendly, silver nanoparticles in quaternized oligochitosan solution was synthesized by using novel oligochitosan derivative with structure of quatemary ammonium salt as reducing agent and stabilizer. Different concentrations of silver nanoparticles in quaternized oligochitosan solution were used to test the resistance against TMV. The results showed that the silver nanoparticles in quaternized oligochitosan solution showed uniform distribution observed under transmission electron microscope (TEM), the particle diameter were mainly 7-12 nm. The best inhibitory effect of silver nanoparticles in quaternized oligochitosan solution to necrosis spots on Nicotiana tabacum cv. Xanthi-nc NN was observed at 25 μ g/mL, with inhibition rate of 74.0%, which was 41.5% and 24.4% higher than that of oligochitosan and ningnanmycin, respectively. On Nicotiana tabacum K326, silver nanoparticles in quaternized oligochitosan solution could relieve the decrease of chlorophyll content, improve the activities of defense enzymes such as superoxide dismutase (SOD), peroxidase (POD) and polyphenoloxidase (PPO) in TMV-inoculated leaves, reduce the content of malondialdehyde (MDA) and accumulate the content of soluble protein. The results suggested that silver nanoparticles in quaternized oligochitosan solution can improve the resistance of tobacco plant against TMV. |
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| Keywords: | quaternized oligochitosan derivative silver nanoparticles synthesis tobacco mosaic virus defense enzymes |
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