| 根癌农杆菌介导绿色荧光蛋白基因转化印度酸桔的研究 |
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| 引用本文： | 石玮,李东栋,邓秀新,伊华林.根癌农杆菌介导绿色荧光蛋白基因转化印度酸桔的研究[J].园艺学报,2002,29(2):109-112. |
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| 作者姓名： | 石玮 李东栋 邓秀新 伊华林 |
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| 作者单位： | (华中农业大学作物遗传改良国家重点实验室, 武汉430070) |
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| 基金项目： | 国家自然科学基金资助项目 ( 39830 2 6 0 ) |
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| 摘 要： | 通过根癌农杆菌介导将绿色荧光蛋白基因转入印度酸桔的胚性愈伤组织中, 经潮霉素筛选,获得抗性愈伤组织, 并再生植株。对这些植株进行GUS 染色、PCR 分析、绿色荧光检测和Sourthern 杂交验证, 结果表明绿色荧光蛋白已经在转基因植株中表达。
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| 关 键 词： | 柑桔 愈伤组织 遗传转化 绿色荧光蛋白基因 根癌农杆菌介导 |
| 文章编号： | 0513-353X(2002)02-0109-04 |
| 修稿时间： | 2001年8月27日 |
The Transformation of gfp in Cleopatra (Citrus reticulata ) Mediated by Agrobacterium tumefaciens |
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| Shi Wei,Li Dongdong,Deng Xiuxin,and Yi Hualin.The Transformation of gfp in Cleopatra (Citrus reticulata ) Mediated by Agrobacterium tumefaciens[J].Acta Horticulturae Sinica,2002,29(2):109-112. |
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| Authors: | Shi Wei Li Dongdong Deng Xiuxin and Yi Hualin |
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| Institution: | ( National Key Laboratory in Genetic Improvement of Crops , Huazhong Agriculture University , Wuhan 430070 , China) |
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| Abstract: | The callus of Cleopatra ( Citrus reticulata ), a citrus rootstock cultivar, was transformed by the strain of Agrobacterium tumefaciens EHA105 carrying gfp gene.The callus of Cleopatra ( Citrus reticulata ) was co-cultivation with A.tumefaciens and selected with hygromycin, as led to the regeneration of resistant embryogenic callus and later to plantlets. The plants were verified GFP-transgenic plants by GUS analysis, GFP-fluorescent detection, PCR amplification and Southern hybridization. |
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| Keywords: | Citrus Callus Green fluorescent protein gene ( gfp ) Genetic transformation Agrobacterium -mediated |
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