| 猪MB基因外显子3的多态性分析 |
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| 引用本文： | 丁国杰,刘娣,戴秀丽,柴华.猪MB基因外显子3的多态性分析[J].安徽农业科学,2007,35(11):3165-3166. |
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| 作者姓名： | 丁国杰 刘娣 戴秀丽 柴华 |
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| 作者单位： | 黑龙江生物制品一厂药品研发中心,黑龙江哈尔滨,150069;黑龙江省农业科学院,黑龙江哈尔滨,150069 |
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| 基金项目： | 黑龙江省科技攻关项目(编号:GB01B104) |
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| 摘 要： | 以MB基因作为影响猪肉肉色的候选基因,利用PCRS-SSCP技术,以野猪、野家杂种猪、民猪、大白猪、长白猪5个猪种为研究对象.根据GenBank上猪的MB序列设计了3对引物,对此基因的3个外显子进行SNPs检测.基因型检测结果表明:MB基因在第1、第2外显子上没有发现多态,在第3外显子上检测到了多态.在第3外显子上的第437 bp处存在一个T→C的碱基突变,该突变未导致氨基酸的改变,属沉默突变.经计算各基因型频率、基因频率和试验统计表明:随野猪、野家杂种猪、民猪、长白猪、大白猪肉色的变浅,等位基因N的基因频率也呈下降趋势.
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| 关 键 词： | 猪 MB基因 SNPs PCR-SSCP 肉色 |
| 文章编号： | 0517-6611（2007）11-03165-02 |
| 收稿时间： | 2007-01-15 |
| 修稿时间： | 2007-01-15 |
Analysis of Polymorphism of MB Genes Exon 3 |
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| DING Guo-jie, et al.Analysis of Polymorphism of MB Genes Exon 3[J].Journal of Anhui Agricultural Sciences,2007,35(11):3165-3166. |
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| Authors: | DING Guo-jie |
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| Institution: | The First Bio-products Manufactory of Heilongjiang Province, Harbin, Heilongjiang 150069 |
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| Abstract: | In this research,the MB genes were taken as the candidate gene which could influence the meat color.The wild boar,the crossbreed of the wild boar and pig,min pig,landrace,double muscle pig and large white pig were taken as the research materials.According to the MB gene sequences of swine in GenBank,three pairs of primers for MB were designed.PCR-SSCP technology was applied to detect SNPs of the genes exons.Detected results of genotype demonstrated that in the first and second primers,there was no polymorphism,and the polymorphism was examined in the third primers of MB.One mutant site(T→C) in the third primer of MB was at 438 bp and the mutation was silent.The computation of genotypic frequency and gene frequency,as well as statistical analysis demonstrated that with the decline of meat color of the wild boar and pig,min pig,landrace,double muscle pig and large white pig,the gene frequency of allelic gene N also declined in this trend. |
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| Keywords: | Pig MB gene SNPs PCR-SSCP Meat color |
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