|
|||||
|
|
| 苏云金杆菌对松墨天牛幼虫的致死作用及其生物学特性 | |
| 引用本文： | 徐华潮,徐金华,张立钦,林海萍,杨萍,程剑明.苏云金杆菌对松墨天牛幼虫的致死作用及其生物学特性[J].林业科学,2010,46(4). |
| 作者姓名： | 徐华潮 徐金华 张立钦 林海萍 杨萍 程剑明 |
| 作者单位： | 1. 浙江林学院林业与生物技术学院,临安,311300 2. 浙江林学院林业与生物技术学院,临安,311300;浙江省淳安县林业局姜家林业中心站,淳安,311712 3. 浙江省现代森林培育技术重点实验室,临安,311300 4. 浙江省淳安县林业局姜家林业中心站,淳安,311712 |
| 基金项目： | 国家948项目(2450000042)资助 |
| 摘 要： | 苏云金杆菌(Bacillus thuringiensis),简称Bt,其在形成芽孢的同时会在菌体的1端或2端形成蛋白晶体,这种蛋白晶体被称为杀虫晶体蛋白(insecticidal crystalproteins,ICPs)或δ-内毒素(delta-endotoxin)。因ICPs具有杀虫高效、广谱、对 |
| 关 键 词： | 苏云金杆菌 松墨天牛 生物防治 松材线虫病 |
Virulence of Bacillus thuringiensis to Monochamus alternatus and Its biological Characteristics |
|
| Xu Huachao,Xu Jinhua,Zhang Liqin,Lin Haiping,Yang Ping,Cheng Jianming.Virulence of Bacillus thuringiensis to Monochamus alternatus and Its biological Characteristics[J].Scientia Silvae Sinicae,2010,46(4). | |
| Authors: | Xu Huachao Xu Jinhua Zhang Liqin Lin Haiping Yang Ping Cheng Jianming |
| Institution: | 1. School of Forestry and Bio-Technology/a>;Zhejiang Forestry University Lin'an 311300/a>;2. Jiangjia Forestry Central Station/a>;Chun'an Forestry Bureau Chun'an 311712/a>;3. Key Laboratory for Modern Silvicultural Technology of Zhejiang Province Lin'an 311300 |
| Abstract: | The virulence of 17 Bacillus thuringiensis strains against 2-3 instar larvae of Monochamus alternatus were tested in indoor.The results showed that two strains (RBT-200701, RBT-200702) exhibited high poisonous activity, and the caused corrected mortality of 2-3 instar M.alternatus larvae were all more than 70%.A positive correlation was found between the corrected mortality and the concentration of spores and parasporal crystal(125-2 000 μg·g-1). The insecticidal effect increased with treatment time (0-20 d).The morphological changes of the two strains at various developmental stages were examined with microscopy on the cell smear.The optimal fermentation condition of strains was 30 ℃, pH7.2,200 r·min-1,for 36 h. The two strains had the same protein spectrum after SDS-PAGE analysis. |
| Keywords: | Bacillus thuringiensis Monochamus alternatus biological control pine wilt disease |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |