| 应用微卫星DNA技术研究中华蜜蜂群内工蜂监督效果 |
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| 引用本文： | 谢宪兵,苏松坤,郑云林,吴小波,曾志将.应用微卫星DNA技术研究中华蜜蜂群内工蜂监督效果[J].中国农业科学,2008,41(6):1816-1821. |
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| 作者姓名： | 谢宪兵 苏松坤 郑云林 吴小波 曾志将 |
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| 作者单位： | 江西农业大学蜜蜂研究所 |
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| 基金项目： | 国家自然科学基金
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泉州师范学院校自选项目 |
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| 摘 要： | 【目的】研究不同中华蜜蜂（Apis cerana cerana）群内的工蜂繁殖现象，探讨蜂群的工蜂监督效果。【方法】以中华蜜蜂为试验材料，对处女蜂王分别进行单雄和双雄人工授精，并以蜂王自然交尾的蜂群作为对照。蜂王成功繁殖后7周，利用蜜蜂微卫星DNA技术检测蜂群内的雄蜂是由蜂王产的未受精卵发育而成，还是由工蜂产的未受精卵发育而成。【结果】所有蜂群中的雄蜂都是由蜂王产的未受精卵发育而成。【结论】在人工授精和自然交尾蜂群中都明显存在工蜂监督行为。
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| 关 键 词： | 中华蜜蜂 工蜂繁殖 工蜂监督 微卫星DNA 人工授精 |
| 收稿时间： | 2007-5-11 |
Study on the Worker Policing in Apis Cerana Cerana Based on Microsatellite DNA |
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| XIE Xian-bing,SU Song-kun,ZHENG Yun-lin,WU Xiao-bo,ZENG Zhi-jiang.Study on the Worker Policing in Apis Cerana Cerana Based on Microsatellite DNA[J].Scientia Agricultura Sinica,2008,41(6):1816-1821. |
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| Authors: | XIE Xian-bing SU Song-kun ZHENG Yun-lin WU Xiao-bo ZENG Zhi-jiang |
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| Abstract: | Abstract: 【Objective】 For exploring the effect of worker policing in Apis cerana cerana, the worker reproduction was studied in different colonies. 【Method】 Apis cerana cerana was the studied biology in this paper. The virgin queens were instrumentally inseminated with 1 or 2 males, and the controlling ones were naturally mated. 7 weeks after the queens reproduced successfully, the drones were detected whether developed from the unfertilized eggs laid by queen or by workers, which based on the technology of honeybee microsatellite DNA that sampled the pupae of worker and drone in every colony. 【Result】 The drones were developed from the unfertilized eggs laid by queen in all of the colonies. 【Conclusion】 The activity of worker policing are taking place universally in colonies which the queens were instrumentally inseminated and naturally mated. |
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| Keywords: | Apis cerana cerana Worker reproduction Worker policing Microsatellite DNA Artificial inseminated |
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