| 两种新疆羊肚菌的分类学鉴定 |
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| 引用本文： | 苏俊,孜来古丽·米吉提,艾尔肯·热合曼,白岚.两种新疆羊肚菌的分类学鉴定[J].新疆农业科学,2011,48(9):1704-1710. |
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| 作者姓名： | 苏俊 孜来古丽·米吉提 艾尔肯·热合曼 白岚 |
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| 作者单位： | 新疆大学生命科学与技术学院,乌鲁木齐830046;山西省左权中学,山西左权032600;新疆大学生命科学与技术学院,乌鲁木齐830046;新疆医科大学基础医学院,乌鲁木齐830011;新疆大学生命科学与技术学院,乌鲁木齐,830046 |
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| 基金项目： | 国家自然科学基金(31060002); 新疆维吾尔自治区科技厅基金(201091236) |
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| 摘 要： | 目的]对新疆霍城县大西沟采集的两种形异羊肚菌(XJURML4和XJURML5)进行分类鉴定.方法]用真菌专一引物ITS1f和ITS4扩增两菌ITS区并进行序列分析.结果]XJURML4 ITS片段1 130 bp( EU086775),XJURML5 ITS片段1 127 bp(EU086776).两菌的ITS2区100;相似,而ITS1区仅有4 bp差异.BLAST比对测序结果发现XJURML4和XJURML5与近缘种宽圆羊肚菌(MorcheLLa esculenta (L.:Fr.)Pers.var.rotunda Pers.:Fr.)的同源性为99.28;和98.65;；NJ法构建进化树分析ITS序列,两菌也跟宽圆羊肚菌类聚在一起.随机引物OPA3进行RAPD扩增,共产生出六条带,二条相同,剩余四条为多态性条带,表明两菌基因组上也有差异.结论]ITS区序列和进化树分析、OPA3 - RAPD标记技术均表明两菌是宽圆羊肚菌种内的新变种,而与传统形态学鉴定结果不一致.
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| 关 键 词： | 羊肚菌 ITS区 RAPD BLAST NJ法进化树 |
| 收稿时间： | 2011-09-25 |
Taxonomic Identification of Two Morchella in Xinjiang |
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| SU Jun,Zilaiguli Mijiti,Erkin Rahman,BAI Lan.Taxonomic Identification of Two Morchella in Xinjiang[J].Xinjiang Agricultural Sciences,2011,48(9):1704-1710. |
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| Authors: | SU Jun Zilaiguli Mijiti Erkin Rahman BAI Lan |
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| Institution: | SU Jun~(1,2),Zilaiguli Mijiti~(1,3),Erkin Rahman~1,BAI Lan~1 (1.College of Life Science and Technology,Xinjiang University,Urumqi,830046,China,2.Zuoquan High School,Shanxi Province,Zuoquan Shanxi 032600,3.Department of Microorganism,The College of Pre - clinine,Xinjiang Medical University,Urumqi 830011,China) |
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| Abstract: | Objective]The purpose of the project was to identify two Morchella(XJURML4 & XJURML5)collected in Daxigou of Huocheng County,Xinjiang by molecular analysis.Method]The fungi common primer pairs ITS If and ITS4 were applied for amplifying internal transcribed spacer regions of two Morchella,and then RAPD technology were used for sequence analysis.Result]ITS regions of XJURML 4 was 1 130 bp and ITS regions of XJURML 5 was 1 127 bp with EU086775 and EU086776 of GenRank accession numbers respectively.The ITS2... |
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| Keywords: | Morchella ITS regions RAPD BLAST neighbor - Joining phylogenetic tree |
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