| 复合垂直流人工湿地基质理化性质对nirS和nirK型反硝化菌多样性的影响 |
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| 引用本文： | 赵伟,朱四喜.复合垂直流人工湿地基质理化性质对nirS和nirK型反硝化菌多样性的影响[J].水生态学杂志,2024,45(2):132-140. |
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| 作者姓名： | 赵伟 朱四喜 |
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| 作者单位： | 贵州民族大学生态环境工程学院,贵州民族大学生态环境工程学院 |
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| 基金项目： | 2018年贵州省科技支撑计划项目（黔科合支撑[2018]2807）；2015年国家自然科学基金（31560107） |
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| 摘 要： | 采用Illumina高通测序技术，探讨喀斯特地区复合垂直流人工湿地基质理化性质对nirS和nirK型反硝化菌多样性的影响。结果表明，复合垂直流人工湿地中4个池子（即一级垂直流下行池、一级垂直流上行池、二级垂直流下行池、二级垂直流上行池）的基质理化性质存在显著差异。其中，pH在中4个池子中均呈中性，DO呈先下降后升高趋势，下行池的基质电导率EC均比上行池要高；TN、TP含量随着人工湿地梯级呈显著下降趋势，未出现厌氧现象；NO3--N和NH4+-N含量在二级垂直流下行池中大量积累并达到峰值。在AlpHa和Beta多样性分析中，nirS、nirK型两类反硝化细菌丰度随着人工湿地梯级呈先降低后升高的趋势，多样性随人工湿地级数逐渐降低，且下行池丰度多样性显著高于上行池。基质中nirS型反硝化细菌鉴定为5门9属，nirK型为6门14属，变形菌门是nirS、nirK型反硝化菌群的共同优势菌门，相对丰度为55.36%~65.72%、30.06%~45.66%。其中，优势菌属为红杆菌属（Rhodobacter）、脱氯单胞菌属（Dechloromonas）、红假单胞菌属（Rhodopseudomonas）、慢生根瘤菌属（Bradyrhizobium）和氏菌属（Bosea）。红杆菌属和脱氯单胞菌属相对丰度在空间上随着人工湿地级数升高总体呈上升趋势，红假单胞菌属、慢生根瘤菌属和氏菌属在空间上随人工湿地级数升高总体呈下降趋势，此2类优势菌属在空间上存在显著差异（P<0.01）。韦恩图表明nirS型反硝化菌群丰度明显高于nirK型，且各级池中反硝化菌群相似度较高。冗余分析表明基质中nirS和nirK反硝化菌群的构建主要受pH、NH4+-N、DO、TP、TN影响，且nirS型对pH，以及nirK型对NH4+-N的响应更为强烈。综上，本文研究了人工湿地nirS、nirK型反硝化菌多样性特征及其与环境因子的关系，为复合垂直流人工湿地高效脱氮提供理论依据。
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| 关 键 词： | 复合垂直流人工湿地 nirS和nirK型 反硝化细菌 Illumina高通量测序 RDA分析 |
| 收稿时间： | 2022/2/1 0:00:00 |
| 修稿时间： | 2022/3/29 0:00:00 |
Effects of substrate physical and chemical properties on diversity characteristics of nirS and nirK denitrifying bacteria in an integrated vertical-flow constructed |
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| Zhao Wei and ZHU Si-xi.Effects of substrate physical and chemical properties on diversity characteristics of nirS and nirK denitrifying bacteria in an integrated vertical-flow constructed[J].Journal of Hydroecology,2024,45(2):132-140. |
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| Authors: | Zhao Wei and ZHU Si-xi |
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| Institution: | College of Eco-Environmental Engineerimg, Guizhou Minzu University, Guiyang , PRChina,College of Eco-Environmental Engineerimg, Guizhou Minzu University, Guiyang , PRChina |
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| Abstract: | Illumina Qualcomm sequencing technique is used to explore the effects of substrate physical and chemical properties on the community diversity of nirS and nirK denitrifying bacteria from an integrated vertical-flow constructed in the karst area. The results showed that there were significant differences in substrate physical and chemical properties of four pools (i.e. primary vertical flow down-flow pool, primary vertical flow upstream pool, secondary vertical flow down-flow pool and secondary vertical flow upstream pool) in the integrated vertical-flow constructed wetland. Among them, pH was neutral in all the four ponds, DO decreased at first and then increased, and the substrate conductivity EC of the downstream pool was higher than that of the upstream pool. The contents of TN and TP decreased significantly with the cascade of constructed wetlands, and there was no anaerobic phenomenon. the contents of NO3--N and NH4+-N accumulated and reached the peak in the secondary vertical flow downstream pool. In the diversity analysis of AlpHa and Beta, the abundance of nirS and nirK denitrifying bacteria decreased at first and then increased with the cascade of constructed wetlands, and the abundance diversity of downstream ponds was significantly higher than that of upstream ponds. The nirS denitrifying bacteria in the matrix were identified as 5 phyla, 9 genera, and 6 phyla, 14 genera. Proteus is the common dominant phylum of nirS and nirK denitrifying bacteria, with a relative abundance of 55.36%~65.72%, 30.06%~45.66%. Among them, the dominant bacteria are Rhodobacter, Dechloromonas, Rhodopseudomonas, Bradyrhizobium and Bosea. The relative abundance of Rhodotorula and dechlorination bacteria increased with the increase of the grade of constructed wetlands, while that of Rhodopseudomonas, Rhizobium and Streptomyces decreased with the increase of the series of constructed wetlands. There were significant differences between the two dominant genera in space (P < 0.01). The Wayne diagram showed that the abundance of denitrifying bacteria of nirS type was significantly higher than that of nirK type, and the similarity of denitrifying bacteria in all levels of ponds was higher. Redundancy analysis showed that the construction of nirS and nirK denitrifying bacteria in the substrate was mainly affected by pH, DO, TP, TN, NH4+-N, and the response of nirS type to pH and nirK type to NH4+-N was stronger. In summary, this paper studied the diversity characteristics of nirS and nirK denitrifying bacteria in the integrated vertical-flow constructed wetland and their relationship with differnet environmental factors, in order to provide a theoretical basis for efficient nitrogen removal in integrated vertical flow constructed wetlands. |
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| Keywords: | a integrated vertical-flow constructed wetland nirS and nirK type denitrifying bacteria Illumina high-throughput sequencing RDA analysis |
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