| 花椰菜雄性不育系组培快繁及无糖培养技术 |
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| 引用本文： | 吴丽芳,蒋亚莲,张艺萍,崔光芬.花椰菜雄性不育系组培快繁及无糖培养技术[J].北方园艺,2009(6). |
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| 作者姓名： | 吴丽芳 蒋亚莲 张艺萍 崔光芬 |
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| 作者单位： | 云南省农业科学院,花卉研究所,云南,昆明,650205 |
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| 基金项目： | 国家科技支撑计划资助项目(2007BAD45B02);;云南省人才培引资助项目(2006PY04) |
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| 摘 要： | 以花椰菜雄性不育亲本的花球为外植体,进行组培快繁及无糖生产技术的研究。结果表明：在MS＋BA 1.0 mg/L＋NAA 0.3 mg/L的培养基上诱导,25 d时不定芽的分化率达到96%,继代增殖在MS＋BA 0.6 mg/L＋NAA 0.1 mg/L的培养基效果最好,增殖率可达4.25倍。无糖组织培养的培养基为MS无机成分＋NAA 0.1,光照4 000 lx,CO2浓度1 000 mL/L,11 d可大量生根。通过上述方法,1个花球,在4个月可生产上万株组培苗,是一种高效快速的方法。
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| 关 键 词： | 花椰菜 雄性不育系 不定芽 继代增殖 无糖组织培养 |
Rapid Propagation and Sugar-free Tissue Culture for Male-sterile Line Cauliflower |
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| WU Li-fang,JIANG Ya-lian,ZHANG Yi-ping,CU Guan-fen.Rapid Propagation and Sugar-free Tissue Culture for Male-sterile Line Cauliflower[J].Northern Horticulture,2009(6). |
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| Authors: | WU Li-fang JIANG Ya-lian ZHANG Yi-ping CU Guan-fen |
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| Institution: | Flower Research Institute of Yunnan Academy of Agricultural Sciences;Kunming;Yunnan 650205 China |
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| Abstract: | Used flower ball of Male-sterile line cauliflower as the explants for tissue culture and sugar-free technological research. The results showed that:when Induced 25 d differentiation rate of adventitious bud in the MS+BA1.0 mg/L+NAA 0.3 mg/L was 96%.The best medium of subculture was MS+BA 0.6 mg/L+NAA 0.1 mg/L. The proliferation rate was 4.25 times. The medium of sugar-free tissue culture was MS inorganic component +NAA 0.1. At light 4 000 lx and CO2 concentrations 1 000 mL/L, 11 d could be substantial root.... |
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| Keywords: | Male-sterile line cauliflower Adventitious buds Subculture proliferation Sugar-free tissue culture |
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