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Merogony in in vitro cultures of Theileria parva
Authors:Madder Maxime  Speybroeck Niko  Berkvens Dirk  Baudoux Valerie  Marcotty Tanguy  Pita Bah Ibrahima  Geysen Dirk  Brandt Jef
Institution:Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerp, Belgium. mmadder@itg.be
Abstract:In vitro studies were focussed on the duration and cessation of merogony in Theileria parva infected blood lymphocyte cell cultures. The cultures were infected using purified tick stabilates as an alternative to in vitro infections, using sporozoites obtained by labour intensive dissections of salivary glands from infected ticks. After establishment of infection in peripheral blood lymphocytes (PBL), merozoites were temporarily produced for about 2 months after which lymphoblasts only contained schizonts.
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