| 茄子SSR多态性标记筛选及杂交种纯度鉴定 |
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| 引用本文： | 张洪源,谈杰,张敏,黄树苹,陈霞,王春丽,谈太明.茄子SSR多态性标记筛选及杂交种纯度鉴定[J].南方农业学报,2017,48(7). |
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| 作者姓名： | 张洪源 谈杰 张敏 黄树苹 陈霞 王春丽 谈太明 |
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| 作者单位： | 武汉市农业科学技术研究院 蔬菜科学研究所,武汉,430065 |
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| 基金项目： | 国家科技支撑计划项目，湖北省科技支撑计划项目 |
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| 摘 要： | 【目的】建立一套快速、准确、高效的茄子杂交种纯度鉴定方法,为其制种过程中除伪存真及大面积推广应用提供技术支持。【方法】挑选已公布的48对SSR引物对2个茄子杂交种15-16和1-7的亲本进行多态性标记筛选,选取有效引物对茄子杂交种进行纯度鉴定,并结合田间植株纯度鉴定,验证SSR分子标记纯度鉴定的有效性。【结果】在48对SSR引物中,有12对引物在杂交种15-16亲本材料中存在多态性,其多态性引物带型呈互补带型的有8对(SM14、SM15、SM17、SM20、SM29、SM30、SM34和SM45);有5对引物在杂交种1-7亲本材料中存在多态性,其多态性引物带型呈互补带型的有4对(SM15、SM20、SM24和SM29)。分别选取2对呈互补带型的SSR引物进行杂交种纯度鉴定,结果显示杂交种15-16和1-7的纯度分别为99%和100%,与田间种植鉴定结果一致。【结论】采用SSR分子标记检测茄子杂交种纯度具有准确、高效的特点,可为茄子杂交制种过程中真假杂种鉴定提供技术支持。
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| 关 键 词： | 茄子 杂交种 纯度鉴定 SSR分子标记 |
Screening of polymorphic SSR markers and hybrid seed purity identification of Solanum melongena L. |
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| ZHANG Hong-yuan,TAN Jie,ZHANG Min,HUANG Shu-ping,CHEN Xia,WANG Chun-li,TAN Tai-ming.Screening of polymorphic SSR markers and hybrid seed purity identification of Solanum melongena L.[J].Journal of Southern Agriculture,2017,48(7). |
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| Authors: | ZHANG Hong-yuan TAN Jie ZHANG Min HUANG Shu-ping CHEN Xia WANG Chun-li TAN Tai-ming |
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| Abstract: | Objective]A fast, accurate and effective method for testing purity of Solanum melongena L. hybrids was established to provide technical support for testing the purity in seed production and promoting its application in large scale.Method]Forty-eight released SSR primers were selected for polymorphisms screening of parents of two eggplant hybrids 15-16 and 1-7. Then, effective primers were selected to identify the purity of S. melongena L. hybrids seeds. Combining field plant purity identification, the validity of purity identification by SSR molecular markers was verified. Result]Among the 48 pairs of primers, 12 pairs showed polymorphism in parent of hybrid 15-16, in which polymorphic primers banding type of 8 pairs was complementary type(SM14, SM15, SM17, SM20, SM29, SM30, SM34 and SM45). Five pairs showed polymorphism in parent of hybrid 1-7, in which polymorphic primers banding type of four pairs was complemen-tary type(SM15, SM20, SM24 and SM29). Two pairs of SSR primers which were complementary types to each other were selected for hybrid purity identification. The results indicated that the purities of hybrid 15-16 and hybrid 1-7 were 99% and 100% respectively, which were in line with field identification. Conclusion]Purity identification of S. melongena L. hybrids seeds by SSR molecular markers is accurate and effective, which can provide technical support in identifying purity of hy-brid seed production. |
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| Keywords: | Solanum melongena L hybrid purity identification SSR molecular marker |
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