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Urease activity in two cultivated and non-cultivated arid soils
Authors:L B Fenn  J L Tipton  G Tatum
Institution:(1) Agricultural Experiment Station, Texas A & M University, 1380 A & M Circle, 79927 El Paso, TX, USA
Abstract:Summary Since urease activity in soil is believed to be relatively constant, the present study was designed to examine the effects of incubation, soil depth and the effect of cultivation on the persistence of urease activity in arid soils. Two soils were used, a Harkey (coarse, silty, mixed, calcareous, thermic, Typic Torrifluvent) and a Saneli (Clayey over sandy skeletal, montmorillonitic, calcareous, Vertic Torrifluvent), each consisting of a cultivated field and a non-cultivated roadbed site. Urease activity was much lower and more varable in the roadbed soils (40 years without cultivation) than in the cultivated field soils. Pre-incubation for 24 h with urea (with toluene) and without urea (without toluene) greatly reduced the total urease activity in all cases in relation to ldquocell freerdquo urease activity (with toluene). Urease activity in the two field soils decreased slightly with profile depth but the decrease was greatest below the plow depth (33 cm). Protease activity or some inactivation processes must have lowered the urease content since there was substantially reduced urease activity after most pre-incubations. The extent of the urease activity decrease was so great that the addition of urea would have been required to increase the production of urease enzyme.
Keywords:Urea fertilization  Urease  Soil depth  Soil organic matter  Arid soils  Pre-incubation
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