| 致犊牛腹泻肠毒素大肠杆菌多重PCR检测方法的建立 |
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| 引用本文： | 段新华,李建军,杨学云,王登峰,王治才.致犊牛腹泻肠毒素大肠杆菌多重PCR检测方法的建立[J].中国动物检疫,2010,27(1):33-34. |
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| 作者姓名： | 段新华 李建军 杨学云 王登峰 王治才 |
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| 作者单位： | 新疆畜牧科学院兽医研究所,新疆乌鲁木齐,830000 |
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| 基金项目： | 自治区十一五重大科技科技专项(200731134)"奶产业技术开发与集成示范"子课题一农区奶牛疾病防治的规范制定和规模化示范 |
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| 摘 要： | 肠毒素大肠杆菌((Ent erot oxi geni c E.col i,ETEC)是引起犊牛腹泻的主要病原之一。本试验建立了多重PCR检测ETEC毒力因子F41菌毛、K99菌毛和STa、LT肠毒素相关基因的技术方法。试验对影响PCR扩增的dNTP、引物浓度以及退火温度等因素进行优化,确定了多重PCR的特异性和灵敏性。结果表明:所建立的多重PCR方法快速、特异、灵敏,在2.5h-3h内就可以完成,为致犊牛腹泻肠毒素大肠杆菌的快速准确检测提供另一种选择。
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| 关 键 词： | 肠毒素大肠杆菌 多重PCR F41菌毛 K99菌毛 STa LT |
Identification of enterotoxigenic Escherichia coli strains causing diarrhea calf by multiplex PCR |
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| Duan Xinhu,Li Jianjun,Yang Xueyun,Wang Dengfeng,Wang Zhicai.Identification of enterotoxigenic Escherichia coli strains causing diarrhea calf by multiplex PCR[J].China Journal Of Animal Quarantine,2010,27(1):33-34. |
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| Authors: | Duan Xinhu Li Jianjun Yang Xueyun Wang Dengfeng Wang Zhicai |
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| Institution: | Duan Xin-hua,Li Jian-jun,Yang Xue-yun,Wang Deng-feng,Wang Zhi-cai,( Institute of Veterinary Medicine,Xinjiang Academy of Animal Science,Xinjiang Urumqi 830000) |
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| Abstract: | Diarrhea in calves is commonly caused by enterotoxigenic Escherichia coli (ETEC). A multiplex PCR method was developed to identify enterotoxigenic Escherichia coli strains by amplifying genes encoding F4l and/or K99 fimbriae,heat-stable enterotoxin a (STa),heat-labile enterotoxin (LT). The parameters of PCR amplification, including concentration of dNTP,primers and annealing temperature were optimized.The specificity and sensitivity of the multiplex PCR were determined on the basis of optimized parameters.I... |
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| Keywords: | STa LT |
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