| 仙鹤草多糖的提取及部分理化性质 |
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| 引用本文： | 张凯,戴玲,岳晓婧.仙鹤草多糖的提取及部分理化性质[J].中国林副特产,2008(5):24-26. |
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| 作者姓名： | 张凯 戴玲 岳晓婧 |
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| 作者单位： | 安徽大学生命科学学院,安徽省中药研究与开发重点实验室,合肥,230039 |
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| 基金项目： | 安徽大学校科研和教改项目 |
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| 摘 要： | 以多糖提取率为指标，采用L9（3^4）正交实验设计对仙鹤草多糖的提取工艺条件进行优化，并采用脱蛋白与DE-52纤维素离子交换柱层析法对仙鹤草多糖进一步分离纯化．同时对多搪的性质进行分析鉴定。实验结果表明，仙鹤草多糖的最佳提取工艺为：浸提温度为80℃，浸提时间12h，料液比1：15．在此条件下仙鹤草多糖提取率为2．15％，浸提温度是影响得率的主要因素。纯化后的仙鹤草多糖组分HAPI、HAPII多糖含量分别为70．08％、78．80％。
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| 关 键 词： | 仙鹤草 多糖 提取工艺 理化性质 |
Extraction and the Physical-chemical Properties of Polysaccharides from Agrimonia pilosa |
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| Zhang Kai,Dai Ling,Yue Xiaojing.Extraction and the Physical-chemical Properties of Polysaccharides from Agrimonia pilosa[J].Forest By-product and Speciality in China,2008(5):24-26. |
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| Authors: | Zhang Kai Dai Ling Yue Xiaojing |
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| Institution: | (College of Life Science, Anhui University , Anhui Key Laboratory of Chinese Medicine Research and Development ,Hefei 230039) |
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| Abstract: | Crude polysaccharides from Agrimonia pilosa Ledeb were extracted by hot water extraction followed by ethanol precipitation. Orthogonal experiment design L9 （3^4）was applied to optimize the extracting process. The polysaccharides were further deproteined by TCA and purified by chromatography on DEAE-cellulose-52 column. Re- sults showed that the optimal extraction parameters were established as follows： temperature 80℃, extracting time 12 hours, solid-liquid ratio 1 ： 15. The yield of the polysaccharides was 2.15%. After deprotein, column chroma- tography purification, main two polysaccharide fractions termed HAPI ＆ HAPII were obtained. Partial physico- chemical properties were investigated by chemical analysis. The content of protein of HAPI ＆ HAPII were 70. 08% ＆78. 80% respectively. |
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| Keywords: | Agrimonia pilosa Ledeb Polysaccharides Extraction technology physicochemical properties |
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