Quantitative counter-immunoelectrophoresis for estimation of antibodies to infectious bursal disease virus |
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| Authors: | G Dhinakar Raj A Thangavelu S Elankumaran A Koteeswaran A T Venugopalan |
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| Institution: | (1) Vaccine Research Centre, Centre for Animal Health Studies, Tamilnadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, 600 051 Madras, India |
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| Abstract: | Quantitative counter-immunoelectrophoresis was standardized to detect antibodies to the avian infectious bursal disease virus. This technique correlated well with the conventional quantitative agar gel precipitation test in estimating antibodies to IBDV. The use of blood dried on filter paper as an alternative to serum is discussed. QCIE is simple, easy to perform and faster than QAGP.Abbreviations AGP
agar gel precipitation
- CID50
half-minimal chick infective dose
- CIE
counter-immunoelectrophoresis
- EEO
electroendosmosis
- IBD
infectious bursal disease
- IBDV
infectious bursal disease virus
- QAGP
quantitative agar gel precipitation
- QCIE
quantitative counter-immunoelectrophoresis |
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| Keywords: | antibody chicken counter-immunoelectrophoresis diagnosis filter paper virus |
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