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Lyophilised bovine serum as a substitute for frozen serum in the cultivation of Babesia bigemina and B bovis.
Authors:L Fish  E Pipano  P Indrakamhang
Institution:Division of Parasitology, Kimron Veterinary Institute, Beit-Dagan, Israel.
Abstract:Lyophilised serum offers significant advantages over frozen serum when it comes to shipping such material over long distances. Babesia bigemina and B bovis were cultured in medium supplemented by either frozen-thawed or lyophilised-rehydrated serum. There were no significant differences between the two types of medium in the growth of parasites and percentage of infected cells during subcultivation for 18 days.
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