| 犬瘟热病毒蓝狐分离株融合蛋白基因的序列分析 |
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| 引用本文： | 苏凤艳,丁庆东,宗春苗,王卓聪,王春雨,王全凯.犬瘟热病毒蓝狐分离株融合蛋白基因的序列分析[J].中国预防兽医学报,2007,29(10):764-767,782. |
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| 作者姓名： | 苏凤艳 丁庆东 宗春苗 王卓聪 王春雨 王全凯 |
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| 作者单位： | 吉林农业大学,中药材学院,吉林,长春,130118 |
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| 摘 要： | 根据已发表的犬瘟热病毒(Canine distemper virus,CDV)Onderstepoort株序列设计1对引物,RT-PCR法扩增出约1 000 bp的融合蛋白基因片段,通过T-A克隆技术,将PCR产物克隆至pMD18-T载体。序列分析表明CDV蓝狐分离株F蛋白基因片段由1 044 bp组成,编码348个氨基酸,与其它CDV25259株、2544-Han95株、A75-17株、DOGDK91C株、5804P株、ONP株、PDV-2株核苷酸序列同源性分别为94.5%、94.1%、94.7%、94.2%、94.2%、97.6%和94.9%;氨基酸序列的同源性分别为98.6%、97.1%、98.0%、98.0%、98.3%、97.7%和98.6%;与其它CDV毒株相比,蓝狐分离株存在核苷酸缺失突变(1 030位～1 038位)和氨基酸缺失突变(344位～348位)。
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| 关 键 词： | 蓝狐 犬瘟热病毒 融合蛋白基因 序列分析 |
| 文章编号： | 1008-0589（2007）10-0764-05 |
| 修稿时间： | 2007-03-15 |
Nucleotide sequence analysis of fusion protein gene of canine distemper virus isolated from alopex |
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| SU Feng-yan,DING Qing-dong,ZONG Chun-miao,WANG Zuo-chong,WANG Chun-yu,WANG Quan-kai.Nucleotide sequence analysis of fusion protein gene of canine distemper virus isolated from alopex[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(10):764-767,782. |
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| Authors: | SU Feng-yan DING Qing-dong ZONG Chun-miao WANG Zuo-chong WANG Chun-yu WANG Quan-kai |
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| Institution: | College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | The fusion protein(F) gene of canine distemper virus(CDV) was amplified by RT-PCR from the genomic RNA isolated from alopex.The PCR product was clones into pMD18-T vector and sequenced.The fusion gene of CDV-A1 strain shared a nucleotide identity of 94.5 %,94.1 %,94.7 %,94.2 %,94.2 %,97.6 %,94.9 % and an amino acid identity of 98.6 %,97.1 %,98.0 %,98.0 %,98.3 %,97.7 %,98.6 % with those of strain 25259,2544Han95,A75-17,DOGDK91C,5804P,ONP,PDV-2,respectively.There was a deletion at nucleotide position of 1 030-1 038 and amino acid position of 344-348 in the F gene of the alopex isolate. |
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| Keywords: | alopex canine distemper virus fusion protein gene sequence analysis |
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