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| 利用透明颤菌血红蛋白基因vgb改造毕赤酵母分泌型表达载体pPIC9K的研究 | |
| 引用本文： | 孟志刚,张锐,陈毓荃,郭三堆.利用透明颤菌血红蛋白基因vgb改造毕赤酵母分泌型表达载体pPIC9K的研究[J].西北农业学报,2005,14(4):166-170. |
| 作者姓名： | 孟志刚 张锐 陈毓荃 郭三堆 |
| 作者单位： | 1. 西北农林科技大学生命科学学院,杨凌,712100;中国农业科学院生物技术研究所,北京,100081 2. 中国农业科学院生物技术研究所,北京,100081 3. 西北农林科技大学生命科学学院,杨凌,712100 |
| 摘 要： | 为了解决酵母发酵时贫氧限制目的蛋白表达量,利用透明颤菌血红蛋白基因vgb设计毕赤酵母分泌 型表达载体pPIC9K-vgb,并以人乳铁蛋白基因为目的基因转入毕赤酵母GS115。研究结果表明,该表达载体具 有转化操作简单、后期工程菌筛选简便、菌体耐低氧能力强、目的蛋白摇瓶表达量高等优点。该表达载体也可 以通过直接替换目的基因而用于其他蛋白表达系统的开发研究。 |
| 关 键 词： | 透明颤菌血红蛋白基因vgb 毕赤酵母 表达载体pPIC9K 人乳铁蛋白 |
| 文章编号： | 1004-1389（2005）04-0066-05 |
| 收稿时间： | 2005-03-23 |
| 修稿时间： | 2005-06-01 |
Modification of Pichia Pastoris' Secretor Vector pPIC9K by Using Vitreoscilla Hemoglobin Gene (vgb) |
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| MENG Zhi-gang,ZHANG Rui,CHEN Yu-quan and GUO San-dui.Modification of Pichia Pastoris'''' Secretor Vector pPIC9K by Using Vitreoscilla Hemoglobin Gene (vgb)[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(4):166-170. | |
| Authors: | MENG Zhi-gang ZHANG Rui CHEN Yu-quan and GUO San-dui |
| Institution: | College of Life Science, Northwest A & F University, Yangling 712100, China;Institute of Biotechnology, Chinese Academy of Agricultural Science, Beijing 100081, China;Institute of Biotechnology, Chinese Academy of Agricultural Science, Beijing 100081, China;College of Life Science, Northwest A & F University, Yangling 712100, China;Institute of Biotechnology, Chinese Academy of Agricultural Science, Beijing 100081, China |
| Abstract: | In order to enhance the yeast ferment expression level of target protein under hypoxic environment, we constructed a new secrctor vector pPIC9K-vgb of Pichia Pastoris by use of Vitreoscilla Hemoglobin Gene (vgb) and the expression vector pPC9K-vgbhLf by used Human Lactoferrin gene (hLf) as target gene . After transgcnic pPIC9K-vgbhLf to the GS115,the results showed that it can simplify screening step of high level expression transformants, enhance anti-hypoxic ability of strain, and increase the expression level of hLf. This vector also can use to study on other proteins by replace the target protein gene. |
| Keywords: | Vitreoscilla Hemoglobin gene Pichia Pastoris Expression vector pPIC9K Human Lactorferrin |
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