高效液相色谱法测定胡椒叶片中6-BA与GA3激素含量 |
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引用本文: | 王丽萍,闫 林,范 睿,黄丽芳,吴 刚.高效液相色谱法测定胡椒叶片中6-BA与GA3激素含量[J].热带作物学报,2012,32(9):64-67. |
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作者姓名: | 王丽萍 闫 林 范 睿 黄丽芳 吴 刚 |
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作者单位: | 中国热带农业科学院热带香料饮料作物研究所/海南省热带香辛饮料作物遗传改良与品质调控重点实验室/农业部香辛饮料作物遗传资源利用重点实验室 海南万宁 571533;中国热带农业科学院热带香料饮料作物研究所/海南省热带香辛饮料作物遗传改良与品质调控重点实验室/农业部香辛饮料作物遗传资源利用重点实验室 海南万宁 571533;中国热带农业科学院热带香料饮料作物研究所/海南省热带香辛饮料作物遗传改良与品质调控重点实验室/农业部香辛饮料作物遗传资源利用重点实验室 海南万宁 571533;中国热带农业科学院热带香料饮料作物研究所/海南省热带香辛饮料作物遗传改良与品质调控重点实验室/农业部香辛饮料作物遗传资源利用重点实验室 海南万宁 571533;中国热带农业科学院热带香料饮料作物研究所/海南省热带香辛饮料作物遗传改良与品质调控重点实验室/农业部香辛饮料作物遗传资源利用重点实验室 海南万宁 571533 |
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摘 要: | 采用Waters nova-pak C18色谱柱(3.9 cm×15 cm,5 μm),87双波长紫外检测器,以甲醇∶乙腈∶磷酸缓冲液(pH 3.5)=15∶15∶70(体积比)为流动相,流速0.8 mL/min,柱温35℃,检测波长210 nm的条件下同时分离测定胡椒叶片中GA3、6-BA植物激素。GA3和6-BA各峰分离效果理想,加标回收率分别为90.80%和88.78%。
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关 键 词: | 高效液相色谱 内源激素 测定 |
Determination of Endogenous Hormones Contents in Piper nigrun cv. Panniyur 1 by High Performance Liquid Chromatography |
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Institution: | Tropical Spice and Beverage Crops Research Institute /Hainan Provincial Key Laboratory for Genetic Improvement and Quality Regulation of TropicalSpice and Beverage Crops / Ministry of Agriculture Key Laboratory for Spice and Beverage Crops Genetic Resourc;Tropical Spice and Beverage Crops Research Institute /Hainan Provincial Key Laboratory for Genetic Improvement and Quality Regulation of TropicalSpice and Beverage Crops / Ministry of Agriculture Key Laboratory for Spice and Beverage Crops Genetic Resourc;Tropical Spice and Beverage Crops Research Institute /Hainan Provincial Key Laboratory for Genetic Improvement and Quality Regulation of TropicalSpice and Beverage Crops / Ministry of Agriculture Key Laboratory for Spice and Beverage Crops Genetic Resourc;Tropical Spice and Beverage Crops Research Institute /Hainan Provincial Key Laboratory for Genetic Improvement and Quality Regulation of TropicalSpice and Beverage Crops / Ministry of Agriculture Key Laboratory for Spice and Beverage Crops Genetic Resourc;Tropical Spice and Beverage Crops Research Institute /Hainan Provincial Key Laboratory for Genetic Improvement and Quality Regulation of TropicalSpice and Beverage Crops / Ministry of Agriculture Key Laboratory for Spice and Beverage Crops Genetic Resourc |
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Abstract: | |
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Keywords: | high performance liquid chromatography endogenous hormones |
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