| 不同生态型云南引种玛咖的多糖含量及多糖纯化工艺研究 |
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| 引用本文： | 高大方,张泽生.不同生态型云南引种玛咖的多糖含量及多糖纯化工艺研究[J].安徽农业科学,2012(36):17756-17757. |
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| 作者姓名： | 高大方 张泽生 |
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| 作者单位： | 1. 天狮集团博士后工作站,天津 301700;天津科技大学食品工程与生物技术学院,天津 301700;天津科技大学博士后流动站,天津 301700
2. 天津科技大学食品工程与生物技术学院,天津,301700 |
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| 摘 要： | 目的]探讨不同生态型玛咖中的活性成分总多糖含量间的差异,并研究玛咖多糖的提取工艺.方法]苯酚-硫酸法分别测定了云南丽江地区引种的4种生态型的玛咖块根中的多糖含量,并进行分析比较.玛咖多糖的基本纯化步骤为水提、醇沉、脱蛋白、脱色.结果]依照颜色不同而划分为4种不同生态型的玛咖中总多糖含量有所不同,几种玛咖块根的总多糖含量顺序为黑色＞紫色＞绿色＞黄色.多糖纯化的最优方法为80％乙醇沉淀、Sevag法脱蛋白.结论]玛咖多糖的含量与其生态型有一定的关系,因此不同生态型的玛咖,其生理活性可能有一定的差别.
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| 关 键 词： | 玛咖 多糖 苯酚-硫酸法 生态型 多糖纯化 |
Comparison of Polysaccharides Content in Different Eco-types of Maca Cultivated in Yunnan and the Purification Process of Polysaccharides in Maca |
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| Institution: | GAO Da-fang et al(Tianshi Group Postdoctoral Workstation,Tianjing 301700) |
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| Abstract: | Objective] To compare the polysaccharide content among four eco-types of cultivated Maca along with its purification technique.Method] The content of polysaccharides in cultivated Maca in Yunnan was determined with Con-H2SO4-phenol spectrophotometry method.The purification of Maca polysaccharides was performed in the procedure of water-extraction,ethanol-precipitation,protein-removal and decoloration.Result] The eco-types of Maca were classified by the color of the rhizomes,so the polysaccharides content detected in different eco-types was arrayed as: black > purple > green > yellow.The optimized purification method of Maca polysaccharide was determined as follows: Extracting with 80% ethanol-water and removing protein with Sevag reagent.Conclusion] The polysaccharide content in Maca has a certain relationship with its eco-types,Maca in different eco-type has differences in biological activity. |
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| Keywords: | Maca Polysaccharides Phenol sulfuric acid method Eco-type Polysaccharide purification |
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