| 纤维素酶产生菌的诱变与筛选 |
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| 引用本文： | 王美珠,曹月坤,陈文艺,姚秀清,李妍妍.纤维素酶产生菌的诱变与筛选[J].安徽农业科学,2012,40(34):16510-16512. |
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| 作者姓名： | 王美珠 曹月坤 陈文艺 姚秀清 李妍妍 |
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| 作者单位： | 辽宁石油化工大学环境与生物工程学院,辽宁抚顺,113001;辽宁石油化工大学石油化工学院,辽宁抚顺,113001 |
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| 摘 要： | 目的]为提高纤维素酶产生菌的酶活力。方法]以抚顺近郊堆积腐烂秸秆的土壤为分离源,利用CMC刚果红平板培养基筛选出纤维素水解圈与菌落直径比值(Hc值)较大的菌株Y-07。以Y-07为出发菌株,在最适诱变剂量条件下,对其进行紫外线诱变和亚硝酸诱变。结果]经过3轮复筛,紫外线诱变后的U10菌株为试验中获得的高产纤维素酶菌株,其酶活最高达到2.499 IU/ml,其活性是Y-07的3.41倍。结论]该方法为进一步提高纤维素酶产生菌的酶活力奠定了基础。
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| 关 键 词： | 纤维素酶 紫外诱变 亚硝酸诱变 酶活 |
Mutation and Screening for Cellulase Production Strain |
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| Institution: | WANG Mei-zhu et al(School of Environment and Bioengineering,Liaoning Shihua University,Fushun,Liaoning 113001) |
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| Abstract: | Objective]The research aimed to improve enzyme activity of cellulase production strain.Method] A strain named Y-07 was isolated from rotten straw soil nearby the suburb of Fushun with culture medium containing the carboxymethylcellulose sodium and Congo Red in accordance with its larger size of ratio of hydrolysis ring to colony diameter.With the strain of Y-07 as starting strain,the strain of Y-07 was mutagenized by ultraviolet radiation and nitrous acid mutagenesis under the most appropriate amount of mutation.Result] After three rounds of secondary screening,the strain of U10 was achieved with the biggest cellulase activity 2.5 IU/ml which was 3.4 times of Y-07 strain.Conclusion]The method provided the basis for further improvements of enzyme activity of cellulase production strain. |
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| Keywords: | Cellulase UV radiation mutagenesis Nitrite mutagenesis Enzyme activity |
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