| 嗜热木聚糖酶酶学性质研究 |
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| 引用本文： | 蒋红宇,申宁,韦萍.嗜热木聚糖酶酶学性质研究[J].安徽农业科学,2012(36):17778-17780. |
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| 作者姓名： | 蒋红宇 申宁 韦萍 |
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| 作者单位： | 南京工业大学,江苏南京,211800 |
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| 摘 要： | 目的]研究嗜热木聚糖酶的酶学性质.方法]考察了基因工程菌Rosetta(DE3)/pET28a-xynB64所产嗜热木聚糖酶反应的最适温度、最适pH,嗜热木聚糖酶的热稳定性、pH稳定性,以及不同金属离子对酶活力的影响.结果]嗜热木聚糖酶的最适反应温度为110℃左右,最适pH为6.6,具有较广的pH稳定范围和非常好的热稳定性,高浓度的Mn2+、Mg2+、Ca2+对该酶有激活作用,Cu2+、Zn2+以及低浓度的Mn2+对该酶有抑制作用.结论]研究可为嗜热木聚糖酶的广泛应用提供参考依据.
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| 关 键 词： | 嗜热木聚糖酶 酶学性质 酶活性 |
Study on the Properties of Thermostable Xylanase |
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| Institution: | JIANG Hong-yu et al(Nanjing University of Technology,Nanjing,Jiangsu 211800) |
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| Abstract: | Objective] To study the properties of thermostable xylanase.Method] The optimal temperature,pH of producing thermostable xylanase by Rosetta(DE3)/pET28a-xynB64,thermal stability,pH stability were investigated,as well as the effects of different metal ions on enzyme activity.Result] The optimal reaction temperature and pH are 110 ℃ and 6.6 with extensive stability range of pH and good thermal stability.High concentration of Mn2+,Mg2+,Ca2+ have activation effect on enzyme,while Cu2+,Zn2+ and low concentration Mn2+ have inhibition effect on the enzyme.Conclusion] The study provides reference basis for extensive application of thermostable xylanase. |
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| Keywords: | Thermostable xylanase Enzymatic properties Enzyme activity |
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