| 亚抑菌浓度穿心莲提取物对减缓金黄色葡萄球菌α-溶血素介导的A549细胞损伤作用的影响 |
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| 引用本文： | 霍明飞,王鑫,戴小寒,邓旭明.亚抑菌浓度穿心莲提取物对减缓金黄色葡萄球菌α-溶血素介导的A549细胞损伤作用的影响[J].中国畜牧兽医,2013,0(5):110-114. |
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| 作者姓名： | 霍明飞 王鑫 戴小寒 邓旭明 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院, 吉林长春 130062;2. 吉林大学军需科技学院, 吉林长春 130062 |
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| 基金项目： | 国家自然基金项目(31072168)。 |
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| 摘 要： | 金黄色葡萄球菌是一种广泛存在的重要致病菌,可引起人类和动物许多严重的感染。另外,α-溶血素是金黄色葡萄球菌最重要的毒力因子之一。本研究通过乙醇回流提取方法,获得中草药穿心莲提取物,然后通过最低抑制浓度(MIC)值测定、溶血活性测定、Western blotting、RT-PCR、LDH及Live/dead等方法研究分析穿心莲提取物对金黄色葡萄球菌α-溶血素分泌的影响及对A549细胞的保护作用。试验结果表明,穿心莲提取物对金黄色葡萄球菌的MIC值为2048 μg/mL,且呈剂量依赖性地抑制金黄色葡萄球菌α-溶血素分泌,并能有效减缓金黄色葡萄球菌菌液上清对A549细胞的损伤。提示,穿心莲提取物是一种潜在的抗金黄色葡萄球菌感染的药物。
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| 关 键 词： | 金黄色葡萄球菌 α-溶血素 穿心莲 |
| 收稿时间： | 2012-10-27 |
Effects of the Subinhibitory Concentrations of Andrographis paniculata Extract on Inhibiting Staphylococcus aureus alpha-hemolysin Mediated A549 Cell Injury |
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| HUO Ming-fei,WANG Xin,DAI Xiao-han,DENG Xu-ming.Effects of the Subinhibitory Concentrations of Andrographis paniculata Extract on Inhibiting Staphylococcus aureus alpha-hemolysin Mediated A549 Cell Injury[J].China Animal Husbandry & Veterinary Medicine,2013,0(5):110-114. |
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| Authors: | HUO Ming-fei WANG Xin DAI Xiao-han DENG Xu-ming |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2. College of Quartermaster Technology, Jilin University, Changchun 130062, China |
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| Abstract: | Staphylococcus aureus was a widely spread opportunistic pathogen with α-hemolysin acting as the vital virulence factor, which could cause serious infection in both human beings and animals. Alcohol reflux was used to obtain the Chinese medicine common Andrographis herb extract. To analyze the effects of the medicine on the α-hemolysin secretion and A549 cell protection, assays including MIC determination, hemolysin assay, Western blotting, RT-PCR, LDH and Live/dead were undertaken. The results showed that the extract could inhibit the hemolysin secretion dose-dependently below the MIC of 2048 μg/mL, and effectively relieve the A549 damage by Staphylococcus aureus supernate, thus indicating it as a potential anti-Staphylococcus aureus drug. |
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| Keywords: | Staphylococcus aureus α-hemolysin Andrographis paniculata |
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