| 抗虫相关基因KTI 对青花菜的转化及其对小菜蛾抗性的分析 |
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| 引用本文： | 江汉民,宋文芹,刘莉莉,文正华,姚星伟,单晓政,孙德岭.抗虫相关基因KTI 对青花菜的转化及其对小菜蛾抗性的分析[J].园艺学报,2013,40(3):498-504. |
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| 作者姓名： | 江汉民 宋文芹 刘莉莉 文正华 姚星伟 单晓政 孙德岭 |
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| 作者单位： | （1 天津科润蔬菜研究所，天津 300384；2 南开大学生命科学学院，天津 300071） |
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| 基金项目： | 现代农业产业技术体系建设专项资金项目，天津市科技支撑计划重点项目 |
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| 摘 要： | 以青花菜（Brassica oleracea L. ssp. italica）下胚轴为外植体，通过农杆菌介导的遗传转化方
法将来源于杨树的Kunitz 型丝氨酸胰蛋白酶抑制剂（KTI）基因导入青花菜品系‘09LR-11’中，获得13
株卡那霉素抗性植株。以KTI 特异引物对转基因植株进行PCR 检测，其中8 株为KTI 阳性植株。Southern
blot 分析进一步表明，基因KTI 已成功整合到青花菜基因组中。RT-PCR 检测表明，KTI 在转基因青花菜
中已成功表达。通过室内叶片离体试验和田间观察，初步证明转KTI 青花菜对小菜蛾幼虫具有一定抗性
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| 关 键 词： | 青花菜 KTI 基因 小菜蛾 抗虫性 转基因 |
Transformation of Broccoli with KTI Gene and the Bioassay for
Diamondback Moth Resistance |
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| JIANG Han-Min,SONG Wen-Qin,LIU Li-Li,WEN Zheng-Hua,YAO Xing-Wei,DAN Xiao-Zheng,SUN De-Ling.Transformation of Broccoli with KTI Gene and the Bioassay for
Diamondback Moth Resistance[J].Acta Horticulturae Sinica,2013,40(3):498-504. |
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| Authors: | JIANG Han-Min SONG Wen-Qin LIU Li-Li WEN Zheng-Hua YAO Xing-Wei DAN Xiao-Zheng SUN De-Ling |
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| Institution: | （1Tianjin Kernel Vegetable Research Institute，Tianjin 300384，China；2College of Life Sciences，Nankai University，
Tianjin 300071，China） |
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| Abstract: | KTI gene was transformed into broccoli（Brassica oleracea L. ssp. italica）genome via
Agrobacterium mediated transformation method using broccoli lines‘09LR-11’as explants. Thirteen
kanamycin resistant broccoli plants were obtained. PCR analysis with primers specific to KTI gene
identified 8 KTI positive plants. Southern blotting analysis with KTI gene as probes showed that the KTI
gene was integrated into broccoli genome. RT-PCR analysis indicated that the KTI gene was expressed in
transgenic broccoli. Both laboratory and field tests proved that the transgenic broccoli plants were resistant
to diamondback moth（Plutella xylostella larvae）. |
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| Keywords: | broccoli KTI gene KTI gene Plutella xylostella larvae insect resistance genetic transformation |
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