| ‘苹果枣’自然三倍体倍性的发现与鉴定 |
| |
| 引用本文： | 刘学生,陈龙,王金鑫,李莉,彭建营.‘苹果枣’自然三倍体倍性的发现与鉴定[J].园艺学报,2013,40(3):426-432. |
| |
| 作者姓名： | 刘学生 陈龙 王金鑫 李莉 彭建营 |
| |
| 作者单位： | 1 河北农业大学园艺学院，河北保定 071001；2 河北省农林科学院昌黎果树研究所，河北昌黎 066600 |
| |
| 基金项目： | 国家自然科学基金项目，河北省自然科学基金项目 |
| |
| 摘 要： | 采用酶解去壁低渗法对枣品种‘苹果枣’进行了核型分析，其核型公式为：2n = 3x = 36 =
21m + 15sm（2SAT），核型为2A 型，是迄今为止新发现的第2 个栆自然三倍体品种。对花粉母细胞减数
分裂观察结果表明，‘苹果枣’分裂不规则，终变期出现单价体、二价体和三价体，其中三价体约占57.5%，
最少含有7 个三价体，最多则含10 个三价体，3 个染色体组之间具有很高的同源性，后期Ⅰ和后期Ⅱ出
现落后染色体和染色体不均等分离现象；末期Ⅱ一些细胞还产生少数三分体及极少数五分体，形成不等
孢子，导致配子体高度不育，由此可判断‘苹果枣’为同源三倍体。用分子标记技术对‘苹果枣’和已
知的三倍体品种‘赞皇大枣’进行分子鉴定，两者分子指纹有显著差异，为不同的两个自然三倍体品种。
|
| 关 键 词： | 枣 自然三倍体 核型 花粉母细胞 减数分裂 |
Discovery and Identification of Natural Triploid Ploidy of Chinese Jujube
Cultivar‘Pingguozao’ |
| |
| LIU Xue-Sheng,CHEN Long,WANG Jin-Xin,LI Li,PENG Jian-Ying.Discovery and Identification of Natural Triploid Ploidy of Chinese Jujube
Cultivar‘Pingguozao’[J].Acta Horticulturae Sinica,2013,40(3):426-432. |
| |
| Authors: | LIU Xue-Sheng CHEN Long WANG Jin-Xin LI Li PENG Jian-Ying |
| |
| Institution: | （1 College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001，China；2 Fruit Institute，Hebei
Academy of Agriculture and Forestry Sciences，Changli，Hebei 066600，China） |
| |
| Abstract: | The methods of eliminating walls by enzymolysis and low osmosis were used to analyze
the chromosome karyotype of Ziziphus jujuba Mill.‘Pingguozao’. The results showed that the karyotype
formula was 2n = 3x = 36 = 21m + 15sm（2SAT），and the karyotype was 2A type. It was second of the
newly discovered natural triploid cultivar. The meiosis course of pollen mother cells of‘Pingguozao’was
observed，and the various periods of the meiosis were described and analyzed. The results showed that the
meiosis of ‘Pingguozao’was irregular. The diakinesis appeared univalent，bivalent and trivalent，and 57.5%
of them were trivalent，at least 7 trivalents，at most contained 10 trivalents. The three genomes had very
high homology. The lagging chromosomes and chromosome unequal separation phenomenon were
observed in anaphase I and II. Some cells also produced few triad and pentad in telophaseⅡ，and formed
unequal spores，and led to highly gametophyte sterility. In view of the above it was deduced that
‘Pingguozao’was autotriploid. The RAPD fingerprints of‘Pingguozao’and‘Zanhuangdazao’were
different，which were two different natural triploid cultivars. |
| |
| Keywords: | Ziziphus jujuba Mill Ziziphus jujuba Mill natural triploid karyotype pollen mother cell meiosis |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
