| 水曲柳体细胞胚与合子胚发生的细胞学研究 |
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| 引用本文： | 孔冬梅,沈海龙,冯丹丹,张莉杰.水曲柳体细胞胚与合子胚发生的细胞学研究[J].林业科学,2006,42(12):130-133,F0003. |
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| 作者姓名： | 孔冬梅 沈海龙 冯丹丹 张莉杰 |
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| 作者单位： | 1. 山西大学生命科学与技术学院,太原030006
2. 东北林业大学林学院,哈尔滨150040 |
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| 基金项目： | 国家自然科学基金，国家自然科学基金 |
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| 摘 要： | 水曲柳(Fraxinus mandshurica)属木犀科(Oleaceae)白蜡树属,是我国东北重要珍贵硬阔树种之一,主要分布于小兴安岭、长白山、辽宁东部山地等地区, 以材质优良而著称.由于长期不合理的采伐利用,目前可利用的资源急剧减少,已被列为国家三级保护植物(傅立国,1992).进行水曲柳体细胞胚胎发生的研究,在资源保护、树种快繁和基因工程育种上有其重要的现实意义.
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| 关 键 词： | 水曲柳 体细胞胚发生 合子胚发生 细胞学观察 |
| 文章编号： | 1001-7488（2006）12-0130-04 |
| 收稿时间： | 2005-11-07 |
| 修稿时间： | 2005-11-07 |
Cytological investigation of somatic and zygotic embryogenesis of Fraxinus mandshurica |
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| Kong Dongmei,Shen Hailong,Feng Dandan,Zhang Lijie.Cytological investigation of somatic and zygotic embryogenesis of Fraxinus mandshurica[J].Scientia Silvae Sinicae,2006,42(12):130-133,F0003. |
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| Authors: | Kong Dongmei Shen Hailong Feng Dandan Zhang Lijie |
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| Institution: | 1. College of Life Science and Technology, Shanxi University Taiyuan 030006; 2. College of Forestry, Northeast Forestry University Harbin 150040 |
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| Abstract: | Maturing seeds were collected from fifty-year-old trees of Fraxinus mandshurica. The cotyledon and hypocotyl zygotic embryos were excised and cultured on 1/2MS media to induce somatic embryos. In cytological study of embryo development, by the way of paraffin, somatic and zygotic embryogenesis was observed and compared under light microscope. The results showed that somatic embryos from zygotic cotyledons originated directly from single epidermis cell. The somatic embryos that indirectly occurred on the embryogenic callus came from its singular surface cell or internal multi_cells. There was an obvious cytological difference between embryogenic and non-embryogenic callus. Somatic and zygotic embryogenesis underwent the similar course, passing through proembryo, heart, torpedo and cotyledonary stages. The important differences in morphogenetic process exited as following: volume of somatic embryos was smaller than its partner at the same development stage; somatic embryos had no obvious suspensors; somatic embryos showed morphologic variations. |
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| Keywords: | Fraxinus mandshurica somatic embryogenesis zygotic embryogenesis cytological investigation |
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