| 三重巢式PCR技术检测抗草甘膦转基因大豆深加工产品 |
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| 引用本文： | 张明辉,高学军,于艳波,秦君,李庆章.三重巢式PCR技术检测抗草甘膦转基因大豆深加工产品[J].农业生物技术学报,2006,14(5):752-756. |
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| 作者姓名： | 张明辉 高学军 于艳波 秦君 李庆章 |
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| 作者单位： | 东北农业大学生命科学与生物技术研究中心,黑龙江省农业生物基因检测中心,哈尔滨,150030 |
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| 基金项目： | 东北农业大学校科研和校改项目 |
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| 摘 要： | 实验以7种具有代表性的含有大豆成分的深加工产品(卵磷脂、大豆蛋白质粉、巧克力饮品、婴儿米粉、大豆粗油、大豆精炼油和大豆色拉油)作为样品进行定性检测，第一轮三重PCR均未能扩增出任何大豆成分，其灵敏度为0.5％。第二轮三重PCR可以扩增出除大豆精炼油和色拉油以外的所有深加工产品的内源基因Lectin、35S-CTP和EPSPS-NOS基因，其检测灵敏度达到0.005％，结果提示三重巢式PCR检测方法适用于大豆深加工产品的定性检测。
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| 关 键 词： | 抗草苷膦转基因大豆 深加工产品 三重巢式PCR 转基因检测 |
| 文章编号： | 1006-0304（2006）05-0752-05 |
| 收稿时间： | 2005-8-30 |
| 修稿时间： | 2005-10-30 |
Detection of Roundup Ready Soybean in Highly Processed Products by Triplex Nested PCR |
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| ZHANG Ming-hui,GAO Xue-jun,YU Yan-bo,QIN Jun,LI Qing-zhang.Detection of Roundup Ready Soybean in Highly Processed Products by Triplex Nested PCR[J].Journal of Agricultural Biotechnology,2006,14(5):752-756. |
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| Authors: | ZHANG Ming-hui GAO Xue-jun YU Yan-bo QIN Jun LI Qing-zhang |
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| Institution: | Research Center of Life Science and Biotechnique, Detecting Center of Agricultural Genetically Modified Organism of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Seven representative highly processed product samples (soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, crude soybean oil, soybean refine oil, soybean salad oil) were detected by the triplex nested PCR. The first triplex PCR could not detect the insert signals in the processed products, and the sensitivity was 0.5%. However, the second triplex PCR could simultaneously detect RR soybean elements, lectin, 35S-CTP and EPSPS-NOS, and a sensitivity was 0.005%. The results indicate that triplex nested PCR is suited to qualitative detection for Roundup ready soybean in highly processed products. |
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| Keywords: | Roundup Ready soybean highly processed products triplex nested PCR genetically modified organism detection |
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