| 喜旱莲子草生防真菌SF-193分生孢子萌发条件及其侵入过程 |
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| 引用本文： | 刘长河,陈志谊,聂亚锋,张荣胜.喜旱莲子草生防真菌SF-193分生孢子萌发条件及其侵入过程[J].中国生物防治,2010,26(2):154-159. |
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| 作者姓名： | 刘长河 陈志谊 聂亚锋 张荣胜 |
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| 作者单位： | 1. 南京农业大学植物保护学院,南京,210095;江苏省农业科学院植物保护研究所,南京,210014
2. 江苏省农业科学院植物保护研究所,南京,210014 |
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| 基金项目： | 江苏省高技术研究(BG2007334) |
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| 摘 要： | 本研究测定了喜旱莲子草生防菌株SF-193分生孢子的最适萌发条件并观察了其叶面侵入过程。结果显示,在20-30℃、pH5-6、空气相对湿度100%时分生孢子能达到最大萌发率;光照对其萌发无显著影响;pH6、RH100%、28℃黑暗条件下培养4h,分生孢子萌发率接近100%。扫描电镜观察结果显示,SF-193分生孢子在接种4h后萌发形成芽管,6h后在叶片表皮细胞表面形成附着胞侵入叶片组织,24h后次生菌丝转由气孔侵入。
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| 关 键 词： | 喜旱莲子草 生防真菌 分生孢子萌发 侵入 |
Conidial Germination and Infection Process of Biocontrol-fungi Strain SF-193 on Alternanthera philoxeroides |
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| LIU Chang-he
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CHEN Zhi-yi
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NIE Ya-feng
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ZHANG Rong-sheng.Conidial Germination and Infection Process of Biocontrol-fungi Strain SF-193 on Alternanthera philoxeroides[J].Chinese Journal of Biological Control,2010,26(2):154-159. |
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| Authors: | LIU Chang-he CHEN Zhi-yi NIE Ya-feng ZHANG Rong-sheng |
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| Institution: | LIU Chang-he1,2,CHEN Zhi-yi1,NIE Ya-feng2,ZHANG Rong-sheng1,2(1.College of Plant Protection,Nanjing Agricutural University,Nanjing 210095,2.Plant Protection Institute,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China) |
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| Abstract: | Conidial germination and infection of SF-193 on Alternanthera philoxeroides were observed with microscope and ultramicroscope.Environmental factors influencing the conidial germination of SF-193 were studied.The optimal germination rate was observed at 30℃,pH 6 and relative humidity 100%.Light intensity didn＇t affect conidial germination obviously.The conidial germination rate reached to nearly 100% at pH 6,RH 100%,28℃ without light.Scanning electronic microscope observation showed that the germination tube formed after 4 h of inoculation.Appressoria were formed by swelling of the apex of germ tubes on the surface of the epidermal cells and invaded into plants after 6 h of inoculation,and the hyphae invaded through stomatal openings after 24 h of inoculation. |
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| Keywords: | Alternanthera philoxeroides biocontrol fungi conidial germination invasion |
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