| 诱导黄瓜直接器官发生主要影响因素的研究 |
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| 引用本文： | 侯爱菊,朱延明,杨爱馥,张彬彬.诱导黄瓜直接器官发生主要影响因素的研究[J].园艺学报,2003,30(1):101-103. |
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| 作者姓名： | 侯爱菊 朱延明 杨爱馥 张彬彬 |
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| 作者单位： | (东北农业大学生命科学学院植物生物工程研究室, 哈尔滨150030) |
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| 摘 要： | 对诱导黄瓜直接器官发生的主要因素进行了探讨。结果表明: 外植体类型、基因型及植物生长调节剂对再生频率均有显著影响。子叶节是最佳的外植体类型; ‘长春密刺’及‘龙杂黄七号’再生频率较高, 分别达到93. 2 %和92. 5 % , 前者每块外植体上的平均芽数(13. 2) 极显著高于后者(3. 1) ; 62BA 单独存在即可诱导产生丛生芽, 0. 5 mg/ mL 最佳。
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| 关 键 词： | 黄瓜 高效再生体系 直接器官发生 芽分化 |
| 文章编号： | 0513-353X(2003)01-0101-03 |
| 收稿时间： | 2002-2-7 |
| 修稿时间： | 2002年2月7日 |
Main Factors Influencing the Frequency of Direct Organogenesis of Cucumber in Vitro |
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| Hou Aiju,Zhu Yanming,Yang Aifu,and Zhang Binbin.Main Factors Influencing the Frequency of Direct Organogenesis of Cucumber in Vitro[J].Acta Horticulturae Sinica,2003,30(1):101-103. |
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| Authors: | Hou Aiju Zhu Yanming Yang Aifu and Zhang Binbin |
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| Institution: | 1. Department of Urinary Surgery,The Second Aff iliated Hospital,Wenzhou Medical Colege,Wenzhou 325000,China;
2. Department of Urinary Surgery,The First Aff iliated Hospital,Medical College;
3. Department of Pharmacology,Colege of Pharmacy,Jinan University,Guangzhou 510632,China |
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| Abstract: | The main factors influencing the frequency of direct organogenesis of cucumber( Cucumis sativus L.)were discussed.The results showed that the types of explants,genotypes and plant growth regulator had significantly effects on regenerating frequency.The optimum explant is cotyledonary nodes.Genotypes'Changchun mici'and'Longzahuang 7'had higher frequency of regeneration which were 93.2% and 92.1% respectively. But the former differentiated much more buds(13.2 per explant)than that of the latter(3.1 per explant). 6 BA can effectively induced buds and the optimum concentration is 0.5mg/L. |
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| Keywords: | Cucumber High frequency regenerated system Direct organogenesis Differentiation rate of buds |
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