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| 根癌农杆菌介导的番茄遗传转化 | |
| 引用本文： | 任永霞,王罡,季静,王萍.根癌农杆菌介导的番茄遗传转化[J].河北北方学院学报(自然科学版),2005,21(5):35-38. |
| 作者姓名： | 任永霞 王罡 季静 王萍 |
| 作者单位： | 1. 河北北方学院牧业工程系,河北,张家口,075000 2. 天津大学农业与生物工程学院,天津,300072 3. 淮海工学院海洋学院,江苏,连云港,222005 |
| 基金项目： | “国家植物转基因技术研究开发与中试基地建设”专项课题（J99-B-001） |
| 摘 要： | 利用根癌农杆菌（Agrobacterium tumefaciens）EHA101携带LycB基因（番茄红素β-环化酶）和潮霉素抗性基因，以东农704、708、709三个番茄品种的子叶、茎段为转化受体对番茄进行遗传转化，经PCR分子检测初步证明，目的基因LyeB已整合进番茄基因组中． |
| 关 键 词： | 根癌农杆菌 番茄 LycB基因 遗传转化 |
| 文章编号： | 1673-1492(2005)05-0035-04 |
| 收稿时间： | 08 28 2005 12:00AM |
| 修稿时间： | 2005年8月28日 |
The Genetic Transformation of Tomato Mediatied by Agrobacterium tumefaciens |
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| REN Yong-xia,WANG Gang,WANG Ping,JI Jing.The Genetic Transformation of Tomato Mediatied by Agrobacterium tumefaciens[J].Journa of Hebei North University:Natural Science Edition,2005,21(5):35-38. | |
| Authors: | REN Yong-xia WANG Gang WANG Ping JI Jing |
| Institution: | 1. Department of Animal Husbandry and Engineering, Hebei North University , Zhangiiakou, Hehei 075000, China;2. School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China; 3. School of Oceanology, Huaihai Institute of Technology, Lianyungang, Jiangsu 222005, China |
| Abstract: | Mediated by Agrobacterium turnefaciens EHA101 with Target LycB gene and resistant gene to Hygromycin, tomato species including Dongnong 704, 708, 709 being used as material and their cotyledons, petiol segments as acceptor, the genetic transformation of tomato was studied. It was prilminarily proved by the PCR examination that target LycB gene was integrated into the genome of tomato. |
| Keywords: | Agrobactorium tumefacien tomato LycB gene genetic transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |